tetano
Editor, Senior Moderator
Microb Pathog. 2017 Dec 19. pii: S0882-4010(17)31284-6. doi: 10.1016/j.micpath.2017.12.050. [Epub ahead of print]
[h=1]Recombinant M2e-HA2 fusion protein induced immunity responses against intranasally administered H9N2 influenza virus.[/h] Golchin M[SUP]1[/SUP], Moghadaszadeh M[SUP]2[/SUP], Tavakkoli H[SUP]3[/SUP], Ghanbarpour R[SUP]1[/SUP], Dastmalchi S[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a highly contagious respiratory tract disease and is considered a serious community health problem. Influenza viruses possess multiple conserved epitopes which are used for designing universal vaccines. To this aim, the gene coding for N-terminal part of M2e (SLLTEVET) and HA2 (GLFGAIAGF), was synthesized, linked by a (Gly4Ser)[SUB]4[/SUB] peptide linker, and cloned into pGS-21a vector. Afterwards, the construct was transferred into E. coli BL21 (DE3) cells to produce the designed antigenic protein called M2e-HA2. Immunization of mice with these peptides significantly induced humoral immune responses against the influenza virus. Three weeks after the last booster, mice were inoculated intranasally with 1 ? 10[SUP]6[/SUP] EID[SUB]50[/SUB] of H9N2 virus. The results indicated that the recombinant M2e-HA2 fusion protein could protect mice against H9N2 virus.
[h=4]KEYWORDS:[/h] Conserved epitopes; Influenza virus; Recombinant protein; Universal vaccine
PMID: 29273512 DOI: 10.1016/j.micpath.2017.12.050
[h=1]Recombinant M2e-HA2 fusion protein induced immunity responses against intranasally administered H9N2 influenza virus.[/h] Golchin M[SUP]1[/SUP], Moghadaszadeh M[SUP]2[/SUP], Tavakkoli H[SUP]3[/SUP], Ghanbarpour R[SUP]1[/SUP], Dastmalchi S[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza is a highly contagious respiratory tract disease and is considered a serious community health problem. Influenza viruses possess multiple conserved epitopes which are used for designing universal vaccines. To this aim, the gene coding for N-terminal part of M2e (SLLTEVET) and HA2 (GLFGAIAGF), was synthesized, linked by a (Gly4Ser)[SUB]4[/SUB] peptide linker, and cloned into pGS-21a vector. Afterwards, the construct was transferred into E. coli BL21 (DE3) cells to produce the designed antigenic protein called M2e-HA2. Immunization of mice with these peptides significantly induced humoral immune responses against the influenza virus. Three weeks after the last booster, mice were inoculated intranasally with 1 ? 10[SUP]6[/SUP] EID[SUB]50[/SUB] of H9N2 virus. The results indicated that the recombinant M2e-HA2 fusion protein could protect mice against H9N2 virus.
[h=4]KEYWORDS:[/h] Conserved epitopes; Influenza virus; Recombinant protein; Universal vaccine
PMID: 29273512 DOI: 10.1016/j.micpath.2017.12.050