tetano
Editor, Senior Moderator
J Immunol. 2016 Nov 23. pii: 1600173. [Epub ahead of print]
[h=1]Roles of Aluminum Hydroxide and Monophosphoryl Lipid A Adjuvants in Overcoming CD4+ T Cell Deficiency To Induce Isotype-Switched IgG Antibody Responses and Protection by T-Dependent Influenza Vaccine.[/h] Ko EJ[SUP]1[/SUP], Lee YT[SUP]1[/SUP], Kim KH[SUP]1[/SUP], Lee Y[SUP]1[/SUP], Jung YJ[SUP]1[/SUP], Kim MC[SUP]1,[/SUP][SUP]2[/SUP], Lee YN[SUP]1,[/SUP][SUP]2[/SUP], Kang T[SUP]1[/SUP], Kang SM[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccine adjuvant effects in the CD4-deficient condition largely remain unknown. We investigated the roles of combined monophosphoryl lipid A (MPL) and aluminum hydroxide (Alum) adjuvant (MPL+Alum) in inducing immunity after immunization of CD4 knockout (CD4KO) and wild-type (WT) mice with T-dependent influenza vaccine. MPL+Alum adjuvant mediated IgG isotype-switched Abs, IgG-secreting cell responses, and protection in CD4KO mice, which were comparable to those in WT mice. In contrast, Alum adjuvant effects were dependent on CD4[SUP]+[/SUP] T cells. MPL+Alum adjuvant was effective in recruiting monocytes and neutrophils as well as in protecting macrophages from Alum-mediated cell loss at the injection site in CD4KO mice. MPL+Alum appeared to attenuate MPL-induced inflammatory responses in WT mice, likely improving the safety. Additional studies in CD4-depleted WT mice and MHC class II KO mice suggest that MHC class II[SUP]+[/SUP] APCs contribute to providing alternative B cell help in the CD4-deficient condition in the context of MPL+Alum-adjuvanted vaccination.
Copyright ? 2016 by The American Association of Immunologists, Inc.
PMID: 27881702 DOI: 10.4049/jimmunol.1600173
[PubMed - as supplied by publisher]
[h=1]Roles of Aluminum Hydroxide and Monophosphoryl Lipid A Adjuvants in Overcoming CD4+ T Cell Deficiency To Induce Isotype-Switched IgG Antibody Responses and Protection by T-Dependent Influenza Vaccine.[/h] Ko EJ[SUP]1[/SUP], Lee YT[SUP]1[/SUP], Kim KH[SUP]1[/SUP], Lee Y[SUP]1[/SUP], Jung YJ[SUP]1[/SUP], Kim MC[SUP]1,[/SUP][SUP]2[/SUP], Lee YN[SUP]1,[/SUP][SUP]2[/SUP], Kang T[SUP]1[/SUP], Kang SM[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccine adjuvant effects in the CD4-deficient condition largely remain unknown. We investigated the roles of combined monophosphoryl lipid A (MPL) and aluminum hydroxide (Alum) adjuvant (MPL+Alum) in inducing immunity after immunization of CD4 knockout (CD4KO) and wild-type (WT) mice with T-dependent influenza vaccine. MPL+Alum adjuvant mediated IgG isotype-switched Abs, IgG-secreting cell responses, and protection in CD4KO mice, which were comparable to those in WT mice. In contrast, Alum adjuvant effects were dependent on CD4[SUP]+[/SUP] T cells. MPL+Alum adjuvant was effective in recruiting monocytes and neutrophils as well as in protecting macrophages from Alum-mediated cell loss at the injection site in CD4KO mice. MPL+Alum appeared to attenuate MPL-induced inflammatory responses in WT mice, likely improving the safety. Additional studies in CD4-depleted WT mice and MHC class II KO mice suggest that MHC class II[SUP]+[/SUP] APCs contribute to providing alternative B cell help in the CD4-deficient condition in the context of MPL+Alum-adjuvanted vaccination.
Copyright ? 2016 by The American Association of Immunologists, Inc.
PMID: 27881702 DOI: 10.4049/jimmunol.1600173
[PubMed - as supplied by publisher]