tetano
Editor, Senior Moderator
Sci Rep
. 2023 Dec 9;13(1):21846.
doi: 10.1038/s41598-023-48581-9. A bead-based multiplex assay covering all coronaviruses pathogenic for humans for sensitive and specific surveillance of SARS-CoV-2 humoral immunity
Daniel Stern[SUP] #[/SUP][SUP] 1 [/SUP], Tanja C Meyer[SUP] #[/SUP][SUP] 2 [/SUP], Fridolin Treindl[SUP] 2 [/SUP], Hans Werner Mages[SUP] 2 [/SUP], Maren Krüger[SUP] 2 [/SUP], Martin Skiba[SUP] 2 [/SUP], Jan Philipp Krüger[SUP] 3 [/SUP], Christian M Zobel[SUP] 4 [/SUP], Maximilian Schreiner[SUP] 4 [/SUP], Marica Grossegesse[SUP] 5 [/SUP], Thomas Rinner[SUP] 5 [/SUP], Caroline Peine[SUP] 6 [/SUP], Anna Stoliaroff-Pépin[SUP] 6 [/SUP], Thomas Harder[SUP] 6 [/SUP], Natalie Hofmann[SUP] 5 [/SUP], Janine Michel[SUP] 5 [/SUP], Andreas Nitsche[SUP] 5 [/SUP], Silke Stahlberg[SUP] 7 [/SUP], Antje Kneuer[SUP] 7 [/SUP], Anna Sandoni[SUP] 7 [/SUP], Ulrike Kubisch[SUP] 7 [/SUP], Martin Schlaud[SUP] 7 [/SUP], Annette Mankertz[SUP] 8 [/SUP], Tatjana Schwarz[SUP] 9 [/SUP], Victor M Corman[SUP] 9 10 11 [/SUP], Marcel A Müller[SUP] 9 [/SUP], Christian Drosten[SUP] 9 [/SUP], Kathrin de la Rosa[SUP] 12 13 [/SUP], Lars Schaade[SUP] 14 [/SUP], Martin B Dorner[SUP] 2 [/SUP], Brigitte G Dorner[SUP] 15 [/SUP]
Affiliations
Serological assays measuring antibodies against SARS-CoV-2 are key to describe the epidemiology, pathobiology or induction of immunity after infection or vaccination. Of those, multiplex assays targeting multiple antigens are especially helpful as closely related coronaviruses or other antigens can be analysed simultaneously from small sample volumes, hereby shedding light on patterns in the immune response that would otherwise remain undetected. We established a bead-based 17-plex assay detecting antibodies targeting antigens from all coronaviruses pathogenic for humans: SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV strains 229E, OC43, HKU1, and NL63. The assay was validated against five commercial serological immunoassays, a commercial surrogate virus neutralisation test, and a virus neutralisation assay, all targeting SARS-CoV-2. It was found to be highly versatile as shown by antibody detection from both serum and dried blot spots and as shown in three case studies. First, we followed seroconversion for all four endemic HCoV strains and SARS-CoV-2 in an outbreak study in day-care centres for children. Second, we were able to link a more severe clinical course to a stronger IgG response with this 17-plex-assay, which was IgG1 and IgG3 dominated. Finally, our assay was able to discriminate recent from previous SARS-CoV-2 infections by calculating the IgG/IgM ratio on the N antigen targeting antibodies. In conclusion, due to the comprehensive method comparison, thorough validation, and the proven versatility, our multiplex assay is a valuable tool for studies on coronavirus serology.
. 2023 Dec 9;13(1):21846.
doi: 10.1038/s41598-023-48581-9. A bead-based multiplex assay covering all coronaviruses pathogenic for humans for sensitive and specific surveillance of SARS-CoV-2 humoral immunity
Daniel Stern[SUP] #[/SUP][SUP] 1 [/SUP], Tanja C Meyer[SUP] #[/SUP][SUP] 2 [/SUP], Fridolin Treindl[SUP] 2 [/SUP], Hans Werner Mages[SUP] 2 [/SUP], Maren Krüger[SUP] 2 [/SUP], Martin Skiba[SUP] 2 [/SUP], Jan Philipp Krüger[SUP] 3 [/SUP], Christian M Zobel[SUP] 4 [/SUP], Maximilian Schreiner[SUP] 4 [/SUP], Marica Grossegesse[SUP] 5 [/SUP], Thomas Rinner[SUP] 5 [/SUP], Caroline Peine[SUP] 6 [/SUP], Anna Stoliaroff-Pépin[SUP] 6 [/SUP], Thomas Harder[SUP] 6 [/SUP], Natalie Hofmann[SUP] 5 [/SUP], Janine Michel[SUP] 5 [/SUP], Andreas Nitsche[SUP] 5 [/SUP], Silke Stahlberg[SUP] 7 [/SUP], Antje Kneuer[SUP] 7 [/SUP], Anna Sandoni[SUP] 7 [/SUP], Ulrike Kubisch[SUP] 7 [/SUP], Martin Schlaud[SUP] 7 [/SUP], Annette Mankertz[SUP] 8 [/SUP], Tatjana Schwarz[SUP] 9 [/SUP], Victor M Corman[SUP] 9 10 11 [/SUP], Marcel A Müller[SUP] 9 [/SUP], Christian Drosten[SUP] 9 [/SUP], Kathrin de la Rosa[SUP] 12 13 [/SUP], Lars Schaade[SUP] 14 [/SUP], Martin B Dorner[SUP] 2 [/SUP], Brigitte G Dorner[SUP] 15 [/SUP]
Affiliations
- PMID: 38071261
- DOI: 10.1038/s41598-023-48581-9
Serological assays measuring antibodies against SARS-CoV-2 are key to describe the epidemiology, pathobiology or induction of immunity after infection or vaccination. Of those, multiplex assays targeting multiple antigens are especially helpful as closely related coronaviruses or other antigens can be analysed simultaneously from small sample volumes, hereby shedding light on patterns in the immune response that would otherwise remain undetected. We established a bead-based 17-plex assay detecting antibodies targeting antigens from all coronaviruses pathogenic for humans: SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV strains 229E, OC43, HKU1, and NL63. The assay was validated against five commercial serological immunoassays, a commercial surrogate virus neutralisation test, and a virus neutralisation assay, all targeting SARS-CoV-2. It was found to be highly versatile as shown by antibody detection from both serum and dried blot spots and as shown in three case studies. First, we followed seroconversion for all four endemic HCoV strains and SARS-CoV-2 in an outbreak study in day-care centres for children. Second, we were able to link a more severe clinical course to a stronger IgG response with this 17-plex-assay, which was IgG1 and IgG3 dominated. Finally, our assay was able to discriminate recent from previous SARS-CoV-2 infections by calculating the IgG/IgM ratio on the N antigen targeting antibodies. In conclusion, due to the comprehensive method comparison, thorough validation, and the proven versatility, our multiplex assay is a valuable tool for studies on coronavirus serology.