tetano
Editor, Senior Moderator
Sci Rep
. 2023 Jan 23;13(1):1263.
doi: 10.1038/s41598-023-28591-3.
Neutralization activity of IgG antibody in COVID‑19‑convalescent plasma against SARS-CoV-2 variants
Kiyoto Tsuchiya[SUP] 1 [/SUP], Kenji Maeda[SUP] 2 [/SUP], Kouki Matsuda[SUP] 2 [/SUP], Yuki Takamatsu[SUP] 2 [/SUP], Noriko Kinoshita[SUP] 3 [/SUP], Satoshi Kutsuna[SUP] 3 [/SUP], Tsunefusa Hayashida[SUP] 4 [/SUP], Hiroyuki Gatanaga[SUP] 4 5 [/SUP], Norio Ohmagari[SUP] 3 [/SUP], Shinichi Oka[SUP] 4 5 [/SUP], Hiroaki Mitsuya[SUP] 6 7 8 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated the anti-SARS-CoV-2 antibody levels, anti-spike (S)-immunoglobulin G (IgG) and anti-nucleocapsid (N)-IgG, and the neutralization activity of IgG antibody in COVID‑19‑convalescent plasma against variants of SARS-CoV-2, alpha, beta, gamma, delta, kappa, omicron and R.1 strains. The study included 30 patients with clinically diagnosed COVID-19. The anti-S-IgG and anti-N-IgG levels ranged from 30.0 to 555.1 and from 10.1 to 752.6, respectively. The neutralization activity (50% inhibition concentration: IC[SUB]50[/SUB]) for the wild-type Wuhan strain ranged from < 6.3 to 81.5 µg/ml. IgG antibodies were > 100 µg/ml in 18 of 30 (60%) subjects infected with the beta variant. The IC[SUB]50[/SUB] values for wild-type and beta variants correlated inversely with anti-S-IgG levels (p < 0.05), but no such correlation was noted with anti-N-IgG. IgG antibodies prevented infectivity and cytopathic effects of six different variants of concern in the cell-based assays of wild-type, alpha, gamma, delta, kappa and R.1 strains, but not that of the beta and omicron strains. IgG is considered the main neutralizing activity in the blood, although other factors may be important in other body tissues.
. 2023 Jan 23;13(1):1263.
doi: 10.1038/s41598-023-28591-3.
Neutralization activity of IgG antibody in COVID‑19‑convalescent plasma against SARS-CoV-2 variants
Kiyoto Tsuchiya[SUP] 1 [/SUP], Kenji Maeda[SUP] 2 [/SUP], Kouki Matsuda[SUP] 2 [/SUP], Yuki Takamatsu[SUP] 2 [/SUP], Noriko Kinoshita[SUP] 3 [/SUP], Satoshi Kutsuna[SUP] 3 [/SUP], Tsunefusa Hayashida[SUP] 4 [/SUP], Hiroyuki Gatanaga[SUP] 4 5 [/SUP], Norio Ohmagari[SUP] 3 [/SUP], Shinichi Oka[SUP] 4 5 [/SUP], Hiroaki Mitsuya[SUP] 6 7 8 [/SUP]
Affiliations
- PMID: 36690803
- DOI: 10.1038/s41598-023-28591-3
Abstract
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated the anti-SARS-CoV-2 antibody levels, anti-spike (S)-immunoglobulin G (IgG) and anti-nucleocapsid (N)-IgG, and the neutralization activity of IgG antibody in COVID‑19‑convalescent plasma against variants of SARS-CoV-2, alpha, beta, gamma, delta, kappa, omicron and R.1 strains. The study included 30 patients with clinically diagnosed COVID-19. The anti-S-IgG and anti-N-IgG levels ranged from 30.0 to 555.1 and from 10.1 to 752.6, respectively. The neutralization activity (50% inhibition concentration: IC[SUB]50[/SUB]) for the wild-type Wuhan strain ranged from < 6.3 to 81.5 µg/ml. IgG antibodies were > 100 µg/ml in 18 of 30 (60%) subjects infected with the beta variant. The IC[SUB]50[/SUB] values for wild-type and beta variants correlated inversely with anti-S-IgG levels (p < 0.05), but no such correlation was noted with anti-N-IgG. IgG antibodies prevented infectivity and cytopathic effects of six different variants of concern in the cell-based assays of wild-type, alpha, gamma, delta, kappa and R.1 strains, but not that of the beta and omicron strains. IgG is considered the main neutralizing activity in the blood, although other factors may be important in other body tissues.