tetano
Editor, Senior Moderator
Sci Rep
. 2023 Jul 8;13(1):11094.
doi: 10.1038/s41598-023-37866-8. Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
Irene Muñoz-Gallego[SUP] 1 2 [/SUP], María Ángeles Meléndez Carmona[SUP] 3 4 [/SUP], Carmen Martín Higuera[SUP] 3 4 [/SUP], Esther Viedma[SUP] 3 4 [/SUP], Rafael Delgado[SUP] 3 4 5 [/SUP], María Dolores Folgueira[SUP] 3 4 5 [/SUP]
Affiliations
The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.
. 2023 Jul 8;13(1):11094.
doi: 10.1038/s41598-023-37866-8. Rapid screening of SARS-CoV-2 variants, a key tool for pandemic surveillance
Irene Muñoz-Gallego[SUP] 1 2 [/SUP], María Ángeles Meléndez Carmona[SUP] 3 4 [/SUP], Carmen Martín Higuera[SUP] 3 4 [/SUP], Esther Viedma[SUP] 3 4 [/SUP], Rafael Delgado[SUP] 3 4 5 [/SUP], María Dolores Folgueira[SUP] 3 4 5 [/SUP]
Affiliations
- PMID: 37422474
- PMCID: PMC10329708
- DOI: 10.1038/s41598-023-37866-8
The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10.8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.