tetano
Editor, Senior Moderator
Sci Rep
. 2024 Apr 16;14(1):8781.
doi: 10.1038/s41598-024-59405-9. SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids
Chiu Wang Chau[SUP] #[/SUP][SUP] 1 [/SUP], Alex To[SUP] #[/SUP][SUP] 1 [/SUP], Rex K H Au-Yeung[SUP] 2 [/SUP], Kaiming Tang[SUP] 3 [/SUP], Yang Xiang[SUP] 1 [/SUP], Degong Ruan[SUP] 1 [/SUP], Lanlan Zhang[SUP] 4 [/SUP], Hera Wong[SUP] 1 [/SUP], Shihui Zhang[SUP] 1 [/SUP], Man Ting Au[SUP] 4 [/SUP], Seok Chung[SUP] 5 [/SUP], Euijeong Song[SUP] 6 [/SUP], Dong-Hee Choi[SUP] 5 [/SUP], Pentao Liu[SUP] 1 7 [/SUP], Shuofeng Yuan[SUP] 3 [/SUP], Chunyi Wen[SUP] 8 [/SUP], Ryohichi Sugimura[SUP] 9 10 [/SUP]
Affiliations
SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages' role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome.
. 2024 Apr 16;14(1):8781.
doi: 10.1038/s41598-024-59405-9. SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids
Chiu Wang Chau[SUP] #[/SUP][SUP] 1 [/SUP], Alex To[SUP] #[/SUP][SUP] 1 [/SUP], Rex K H Au-Yeung[SUP] 2 [/SUP], Kaiming Tang[SUP] 3 [/SUP], Yang Xiang[SUP] 1 [/SUP], Degong Ruan[SUP] 1 [/SUP], Lanlan Zhang[SUP] 4 [/SUP], Hera Wong[SUP] 1 [/SUP], Shihui Zhang[SUP] 1 [/SUP], Man Ting Au[SUP] 4 [/SUP], Seok Chung[SUP] 5 [/SUP], Euijeong Song[SUP] 6 [/SUP], Dong-Hee Choi[SUP] 5 [/SUP], Pentao Liu[SUP] 1 7 [/SUP], Shuofeng Yuan[SUP] 3 [/SUP], Chunyi Wen[SUP] 8 [/SUP], Ryohichi Sugimura[SUP] 9 10 [/SUP]
Affiliations
- PMID: 38627497
- PMCID: PMC11021416
- DOI: 10.1038/s41598-024-59405-9
SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages' role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome.