tetano
Editor, Senior Moderator
Sci Rep
. 2021 Oct 6;11(1):19817.
doi: 10.1038/s41598-021-99344-3.
Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
Leonardo Mastropasqua[SUP] #[/SUP][SUP] 1 [/SUP], Lisa Toto[SUP] #[/SUP][SUP] 1 [/SUP], Luigi Chiricosta[SUP] #[/SUP][SUP] 2 [/SUP], Francesca Diomede[SUP] #[/SUP][SUP] 3 [/SUP], Agnese Gugliandolo[SUP] 2 [/SUP], Serena Silvestro[SUP] 2 [/SUP], Guya Diletta Marconi[SUP] 3 [/SUP], Bruna Sinjari[SUP] 4 [/SUP], Jacopo Vecchiet[SUP] 5 [/SUP], Francesco Cipollone[SUP] 6 [/SUP], Damiano D'Ardes[SUP] 6 [/SUP], Antonio Auricchio[SUP] 5 [/SUP], Manuela Lanzini[SUP] 1 [/SUP], Sergio Caputi[SUP] 3 [/SUP], Rossella D'Aloisio[SUP] 7 [/SUP], Emanuela Mazzon[SUP] 2 [/SUP], Oriana Trubiani[SUP] 3 [/SUP]
Affiliations
Abstract
Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction. The main aim of this study was to analyze mRNA expression in the tears of patients with COVID-19 compared with healthy subjects using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 25 genes that differ statistically between healthy individuals and patients affected by COVID-19. In particular, the NGS analysis identified the presence of several genes involved in B cell signaling and keratinization. In particular, the genes involved in B cell signaling were downregulated in the tears of COVID-19 patients, while those involved in keratinization were upregulated. The results indicated that SARS-CoV-2 may induce a process of ocular keratinization and a defective B cell response.
. 2021 Oct 6;11(1):19817.
doi: 10.1038/s41598-021-99344-3.
Transcriptomic analysis revealed increased expression of genes involved in keratinization in the tears of COVID-19 patients
Leonardo Mastropasqua[SUP] #[/SUP][SUP] 1 [/SUP], Lisa Toto[SUP] #[/SUP][SUP] 1 [/SUP], Luigi Chiricosta[SUP] #[/SUP][SUP] 2 [/SUP], Francesca Diomede[SUP] #[/SUP][SUP] 3 [/SUP], Agnese Gugliandolo[SUP] 2 [/SUP], Serena Silvestro[SUP] 2 [/SUP], Guya Diletta Marconi[SUP] 3 [/SUP], Bruna Sinjari[SUP] 4 [/SUP], Jacopo Vecchiet[SUP] 5 [/SUP], Francesco Cipollone[SUP] 6 [/SUP], Damiano D'Ardes[SUP] 6 [/SUP], Antonio Auricchio[SUP] 5 [/SUP], Manuela Lanzini[SUP] 1 [/SUP], Sergio Caputi[SUP] 3 [/SUP], Rossella D'Aloisio[SUP] 7 [/SUP], Emanuela Mazzon[SUP] 2 [/SUP], Oriana Trubiani[SUP] 3 [/SUP]
Affiliations
- PMID: 34615949
- DOI: 10.1038/s41598-021-99344-3
Abstract
Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction. The main aim of this study was to analyze mRNA expression in the tears of patients with COVID-19 compared with healthy subjects using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 25 genes that differ statistically between healthy individuals and patients affected by COVID-19. In particular, the NGS analysis identified the presence of several genes involved in B cell signaling and keratinization. In particular, the genes involved in B cell signaling were downregulated in the tears of COVID-19 patients, while those involved in keratinization were upregulated. The results indicated that SARS-CoV-2 may induce a process of ocular keratinization and a defective B cell response.