tetano
Editor, Senior Moderator
Sci Total Environ
. 2022 Dec 6;160700.
doi: 10.1016/j.scitotenv.2022.160700. Online ahead of print.
Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
Maryia Drobysh[SUP] 1 [/SUP], Arunas Ramanavicius[SUP] 2 [/SUP], Ausra Baradoke[SUP] 3 [/SUP]
Affiliations
Abstract
In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). The affinity interaction between immobilized SCoV2-rS protein and antibodies in the physiological range of concentrations was registered by electrochemical impedance spectroscopy. Analytical parameters of the sensing platform were tuned by the variation of electropolymerization times during the synthesis of PANI-PA films. The lowest limit of detection and quantitation were obtained for electropolymerization time of 20 min and equalled 8.00 ± 0.20 nM and 23.93 ± 0.60 nM with an equilibrium dissociation constant of 3 nM. The presented sensing system is label-free and suitable for the direct detection of antibodies against SARS-CoV-2 in real patient serum samples after coronavirus disease 2019 and/or vaccination.
Keywords: Electrochemical impedance spectroscopy (EIS); Immunosensor; Phytic acid; Polyaniline; SARS-CoV-2 Spike protein; Screen-printed electrodes.
. 2022 Dec 6;160700.
doi: 10.1016/j.scitotenv.2022.160700. Online ahead of print.
Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
Maryia Drobysh[SUP] 1 [/SUP], Arunas Ramanavicius[SUP] 2 [/SUP], Ausra Baradoke[SUP] 3 [/SUP]
Affiliations
- PMID: 36493838
- DOI: 10.1016/j.scitotenv.2022.160700
Abstract
In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). The affinity interaction between immobilized SCoV2-rS protein and antibodies in the physiological range of concentrations was registered by electrochemical impedance spectroscopy. Analytical parameters of the sensing platform were tuned by the variation of electropolymerization times during the synthesis of PANI-PA films. The lowest limit of detection and quantitation were obtained for electropolymerization time of 20 min and equalled 8.00 ± 0.20 nM and 23.93 ± 0.60 nM with an equilibrium dissociation constant of 3 nM. The presented sensing system is label-free and suitable for the direct detection of antibodies against SARS-CoV-2 in real patient serum samples after coronavirus disease 2019 and/or vaccination.
Keywords: Electrochemical impedance spectroscopy (EIS); Immunosensor; Phytic acid; Polyaniline; SARS-CoV-2 Spike protein; Screen-printed electrodes.