tetano
Editor, Senior Moderator
Virology. 2017 Jul 27;510:252-261. doi: 10.1016/j.virol.2017.07.030. [Epub ahead of print]
[h=1]Selection of antigenic variants of an H5N1 highly pathogenic avian influenza virus in vaccinated chickens.[/h] Nguyen LT[SUP]1[/SUP], Nishi T[SUP]2[/SUP], Shichinohe S[SUP]2[/SUP], Chu DH[SUP]2[/SUP], Hiono T[SUP]2[/SUP], Matsuno K[SUP]3[/SUP], Okamatsu M[SUP]2[/SUP], Kida H[SUP]4[/SUP], Sakoda Y[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccination-primed immunity in poultry has been suggested for selection of antigenically drifted highly pathogenic avian influenza viruses (HPAIVs). In this study, we performed two consecutive passage studies of an H5N1 HPAIV in vaccinated chickens, namely, study-I and study-II, to select antigenic variants under immune pressure from the vaccination. In study-I, nine consecutive passages of a wild-type H5N1 HPAIV were carried out in chickens vaccinated with the homologous challenge strain. Antigenically drifted variants with mutations at position 179 in the hemagglutinin (HA) were selected after three passages. Similarly, in study-II, a vaccination-mediated antigenic variant isolated in study-I was used as the vaccine and challenge strain to confirm further antigenic drift after updating the vaccine; after the third passage, additional antigenic variants with a mutation at position 256 in the HA were selected. Thus, our study demonstrated the contribution of vaccination in the selection of antigenic variants of H5 HPAIVs in chickens.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic drift; Chickens; H5; Highly pathogenic avian influenza virus; Vaccine
PMID: 28756116 DOI: 10.1016/j.virol.2017.07.030
[h=1]Selection of antigenic variants of an H5N1 highly pathogenic avian influenza virus in vaccinated chickens.[/h] Nguyen LT[SUP]1[/SUP], Nishi T[SUP]2[/SUP], Shichinohe S[SUP]2[/SUP], Chu DH[SUP]2[/SUP], Hiono T[SUP]2[/SUP], Matsuno K[SUP]3[/SUP], Okamatsu M[SUP]2[/SUP], Kida H[SUP]4[/SUP], Sakoda Y[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Vaccination-primed immunity in poultry has been suggested for selection of antigenically drifted highly pathogenic avian influenza viruses (HPAIVs). In this study, we performed two consecutive passage studies of an H5N1 HPAIV in vaccinated chickens, namely, study-I and study-II, to select antigenic variants under immune pressure from the vaccination. In study-I, nine consecutive passages of a wild-type H5N1 HPAIV were carried out in chickens vaccinated with the homologous challenge strain. Antigenically drifted variants with mutations at position 179 in the hemagglutinin (HA) were selected after three passages. Similarly, in study-II, a vaccination-mediated antigenic variant isolated in study-I was used as the vaccine and challenge strain to confirm further antigenic drift after updating the vaccine; after the third passage, additional antigenic variants with a mutation at position 256 in the HA were selected. Thus, our study demonstrated the contribution of vaccination in the selection of antigenic variants of H5 HPAIVs in chickens.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic drift; Chickens; H5; Highly pathogenic avian influenza virus; Vaccine
PMID: 28756116 DOI: 10.1016/j.virol.2017.07.030