tetano
Editor, Senior Moderator
Signal Transduct Target Ther
. 2021 Sep 16;6(1):342.
doi: 10.1038/s41392-021-00753-7.
Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients
Xiang-Na Zhao[SUP] #[/SUP][SUP] 1 [/SUP], Yue You[SUP] #[/SUP][SUP] 2 3 [/SUP], Xiao-Ming Cui[SUP] #[/SUP][SUP] 4 [/SUP], Hui-Xia Gao[SUP] #[/SUP][SUP] 5 [/SUP], Guo-Lin Wang[SUP] 4 [/SUP], Sheng-Bo Zhang[SUP] 2 3 [/SUP], Lin Yao[SUP] 4 [/SUP], Li-Jun Duan[SUP] 4 [/SUP], Ka-Li Zhu[SUP] 4 [/SUP], Yu-Ling Wang[SUP] 5 [/SUP], Li Li[SUP] 5 [/SUP], Jian-Hua Lu[SUP] 5 [/SUP], Hai-Bin Wang[SUP] 5 [/SUP], Jing-Fang Fan[SUP] 5 [/SUP], Huan-Wei Zheng[SUP] 5 [/SUP], Er-Hei Dai[SUP] 6 [/SUP], Lu-Yi Tian[SUP] 7 8 [/SUP], Mai-Juan Ma[SUP] 9 [/SUP]
Affiliations
Abstract
While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected individuals are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. We performed single-cell transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, and severe patients with healthy controls. Asymptomatic patients displayed increased CD56[SUP]bri[/SUP]CD16[SUP]-[/SUP] natural killer (NK) cells and upregulation of interferon-gamma in effector CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells and NK cells. They showed more robust TCR clonal expansion, especially in effector CD4[SUP]+[/SUP] T cells, but lack strong BCR clonal expansion compared to moderate patients. Moreover, asymptomatic patients have lower interferon-stimulated genes (ISGs) expression in general but large interpatient variability, whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease. Our data provide evidence of different immune signatures to SARS-CoV-2 in asymptomatic infections.
. 2021 Sep 16;6(1):342.
doi: 10.1038/s41392-021-00753-7.
Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients
Xiang-Na Zhao[SUP] #[/SUP][SUP] 1 [/SUP], Yue You[SUP] #[/SUP][SUP] 2 3 [/SUP], Xiao-Ming Cui[SUP] #[/SUP][SUP] 4 [/SUP], Hui-Xia Gao[SUP] #[/SUP][SUP] 5 [/SUP], Guo-Lin Wang[SUP] 4 [/SUP], Sheng-Bo Zhang[SUP] 2 3 [/SUP], Lin Yao[SUP] 4 [/SUP], Li-Jun Duan[SUP] 4 [/SUP], Ka-Li Zhu[SUP] 4 [/SUP], Yu-Ling Wang[SUP] 5 [/SUP], Li Li[SUP] 5 [/SUP], Jian-Hua Lu[SUP] 5 [/SUP], Hai-Bin Wang[SUP] 5 [/SUP], Jing-Fang Fan[SUP] 5 [/SUP], Huan-Wei Zheng[SUP] 5 [/SUP], Er-Hei Dai[SUP] 6 [/SUP], Lu-Yi Tian[SUP] 7 8 [/SUP], Mai-Juan Ma[SUP] 9 [/SUP]
Affiliations
- PMID: 34531370
- PMCID: PMC8443960
- DOI: 10.1038/s41392-021-00753-7
Abstract
While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected individuals are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. We performed single-cell transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, and severe patients with healthy controls. Asymptomatic patients displayed increased CD56[SUP]bri[/SUP]CD16[SUP]-[/SUP] natural killer (NK) cells and upregulation of interferon-gamma in effector CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells and NK cells. They showed more robust TCR clonal expansion, especially in effector CD4[SUP]+[/SUP] T cells, but lack strong BCR clonal expansion compared to moderate patients. Moreover, asymptomatic patients have lower interferon-stimulated genes (ISGs) expression in general but large interpatient variability, whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease. Our data provide evidence of different immune signatures to SARS-CoV-2 in asymptomatic infections.