tetano
Editor, Senior Moderator
Front Immunol. 2017 Nov 27;8:1649. doi: 10.3389/fimmu.2017.01649. eCollection 2017.
[h=1]Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses.[/h] Wu P[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Lu J[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Zhang X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Mei M[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Feng L[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Peng D[SUP]3,[/SUP][SUP]4[/SUP], Hou J[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Kang SM[SUP]5[/SUP], Liu X[SUP]3,[/SUP][SUP]4[/SUP], Tang Y[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective H5 subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) in the baculovirus-insect cell system. The efficacy of the rHA VLPs vaccine with or without immunopotentiator (CVCVA5) was assessed in chickens. Compared to the commercial Re6 or Re6-CVCVA5 vaccines, single dose immunization of chickens with rHA VLPs or rHA-CVCVA5 vaccines induced higher levels of serum hemagglutinin inhibition titers and neutralization titers, mucosal antibodies, IFN-γ and IL-4 cytokines in sera, and cytotoxic T lymphocyte responses. The rHA VLPs vaccine was superior to the commercial Re6 vaccine in conferring cross-protection against different clades of H5 subtype viruses. This study reports that H5 subtype consensus HA VLP single dose vaccination provides broad protection against HPAI virus in chickens.
[h=4]KEYWORDS:[/h] H5 subtype influenza virus; broad protection; consensus; vaccine; virus-like particles
PMID: 29230222 PMCID: PMC5711813 DOI: 10.3389/fimmu.2017.01649
[h=1]Single Dose of Consensus Hemagglutinin-Based Virus-Like Particles Vaccine Protects Chickens against Divergent H5 Subtype Influenza Viruses.[/h] Wu P[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Lu J[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Zhang X[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Mei M[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Feng L[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Peng D[SUP]3,[/SUP][SUP]4[/SUP], Hou J[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Kang SM[SUP]5[/SUP], Liu X[SUP]3,[/SUP][SUP]4[/SUP], Tang Y[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective H5 subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) in the baculovirus-insect cell system. The efficacy of the rHA VLPs vaccine with or without immunopotentiator (CVCVA5) was assessed in chickens. Compared to the commercial Re6 or Re6-CVCVA5 vaccines, single dose immunization of chickens with rHA VLPs or rHA-CVCVA5 vaccines induced higher levels of serum hemagglutinin inhibition titers and neutralization titers, mucosal antibodies, IFN-γ and IL-4 cytokines in sera, and cytotoxic T lymphocyte responses. The rHA VLPs vaccine was superior to the commercial Re6 vaccine in conferring cross-protection against different clades of H5 subtype viruses. This study reports that H5 subtype consensus HA VLP single dose vaccination provides broad protection against HPAI virus in chickens.
[h=4]KEYWORDS:[/h] H5 subtype influenza virus; broad protection; consensus; vaccine; virus-like particles
PMID: 29230222 PMCID: PMC5711813 DOI: 10.3389/fimmu.2017.01649