tetano
Editor, Senior Moderator
J Phys Condens Matter. 2018 Apr 6. doi: 10.1088/1361-648X/aabc21. [Epub ahead of print]
[h=1]Single-particle fusion of influenza viruses reveals complex interactions with target membranes.[/h] van der Borg G[SUP]1[/SUP], Braddock S[SUP]1[/SUP], Blijleven J[SUP]1[/SUP], van Oijen A[SUP]2[/SUP], Roos W[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The first steps in infection of influenza A virus is contact with the host cell membrane, with which it later fuses. The composition of the target bilayer exerts a complex influence on both fusion efficiency and time. Here, an in vitro, single-particle approach is used to study this effect. Using total internal reflection fluorescence (TIRF) microscopy and a microfluidic flow cell, the hemifusion of single virions is visualized. Hemifusion efficiency and kinetics are studied while altering target bilayer cholesterol content and sialic-acid donor. Cholesterol ratios tested were 0%, 10%, 20%, and 40%. Sialic-acid donors GD1a and GYPA were used. Both cholesterol ratio and sialic-acid donors proved to have a significant effect on hemifusion efficiency. Furthermore, comparison between GD1a and GYPA conditions shows that the cholesterol dependence of the hemifusion time is severely affected by the sialic-acid donor. Only GD1a shows a clear increasing trend in hemifusion efficiency and time with increasing cholesterol concentration of the target bilayer with maximum rates for GD1A and 40% cholesterol. Overall our results show that sialic acid donor and target bilayer composition should be carefully chosen, depending on the desired hemifusion time and efficiency in the experiment.
[h=4]KEYWORDS:[/h] Hemifusion; Influenza; Membrane; Membrane Fusion; Sialic-acid; cholesterol; fusion
PMID: 29623903 DOI: 10.1088/1361-648X/aabc21
[h=1]Single-particle fusion of influenza viruses reveals complex interactions with target membranes.[/h] van der Borg G[SUP]1[/SUP], Braddock S[SUP]1[/SUP], Blijleven J[SUP]1[/SUP], van Oijen A[SUP]2[/SUP], Roos W[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The first steps in infection of influenza A virus is contact with the host cell membrane, with which it later fuses. The composition of the target bilayer exerts a complex influence on both fusion efficiency and time. Here, an in vitro, single-particle approach is used to study this effect. Using total internal reflection fluorescence (TIRF) microscopy and a microfluidic flow cell, the hemifusion of single virions is visualized. Hemifusion efficiency and kinetics are studied while altering target bilayer cholesterol content and sialic-acid donor. Cholesterol ratios tested were 0%, 10%, 20%, and 40%. Sialic-acid donors GD1a and GYPA were used. Both cholesterol ratio and sialic-acid donors proved to have a significant effect on hemifusion efficiency. Furthermore, comparison between GD1a and GYPA conditions shows that the cholesterol dependence of the hemifusion time is severely affected by the sialic-acid donor. Only GD1a shows a clear increasing trend in hemifusion efficiency and time with increasing cholesterol concentration of the target bilayer with maximum rates for GD1A and 40% cholesterol. Overall our results show that sialic acid donor and target bilayer composition should be carefully chosen, depending on the desired hemifusion time and efficiency in the experiment.
[h=4]KEYWORDS:[/h] Hemifusion; Influenza; Membrane; Membrane Fusion; Sialic-acid; cholesterol; fusion
PMID: 29623903 DOI: 10.1088/1361-648X/aabc21