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Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies

tetano

Editor, Senior Moderator
Sensors (Basel). 2018 Dec 23;19(1). pii: E52. doi: 10.3390/s19010052.
[h=1]Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies.[/h] Jeong HJ[SUP]1[/SUP], Dong J[SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Ueda H[SUP]5[/SUP].
[h=3]Author information[/h]

[h=3]Abstract[/h] We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.


[h=4]KEYWORDS:[/h] H1N1; Quenchbody; hemagglutinin; immunosensor; influenza A

PMID: 30583603 DOI: 10.3390/s19010052
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