tetano
Editor, Senior Moderator
Spectrochim Acta A Mol Biomol Spectrosc
. 2023 Aug 18;303:123275.
doi: 10.1016/j.saa.2023.123275. Online ahead of print. Directly and ultrasensitivity detecting SARS-CoV-2 spike protein in pharyngeal swab solution by using SERS-based biosensor
Yansheng Liu[SUP] 1 [/SUP], Zhenle Qin[SUP] 2 [/SUP], Xiaobo Jia[SUP] 2 [/SUP], Jin Zhou[SUP] 2 [/SUP], Hongli Li[SUP] 2 [/SUP], Xiaohong Wang[SUP] 2 [/SUP], Yating Chen[SUP] 2 [/SUP], Junpeng Deng[SUP] 2 [/SUP], Zhicheng Jin[SUP] 3 [/SUP], Guofu Wang[SUP] 2 [/SUP]
Affiliations
The global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a great disaster to the economy and human society. Nowadays, SARS-CoV-2 is fading away from people's memory but it still exists around us. PCR plays an important role in detecting SARS-CoV-2 but it requires a long detecting time, equipped laboratory, and professional operators. In comparison with polymerase chain reaction (PCR), surface-enhanced Raman scattering (SERS) is a promising method for detecting SARS-CoV-2 due to its fast, easily operated, and high-sensitivity properties. In this study, the monolayer Ag nanoparticles (MAgNPs) covered with single-layer graphene (SLG) are applied as a SERS substrate. The angiotensin converting enzyme 2 (ACE2) is selected as a bio-probes that can specifically bind to the SARS-CoV-2 S protein. The SERS-based biosensor is formed by ACE2 functionalized SLG/MAgNPs and the LODs of detecting SARS-CoV-2 S protein in phosphate-buffered saline (PBS) and in pharyngeal swabs solution (PSS) are 0.1 fg mL[SUP]-1[/SUP] and 10 fg mL[SUP]-1[/SUP], respectively. This biosensor provides a way of directly detecting SARS-CoV-2 S protein with high sensitivity and specificity. It illustrates a practical potential in the rapid detection of the SARS-CoV-2 virus.
Keywords: ACE2 bio-probe; Biosensor; Monolayer Ag nanoparticles; SARS-CoV-2 S protein; Surface-enhanced Raman scattering (SERS).
. 2023 Aug 18;303:123275.
doi: 10.1016/j.saa.2023.123275. Online ahead of print. Directly and ultrasensitivity detecting SARS-CoV-2 spike protein in pharyngeal swab solution by using SERS-based biosensor
Yansheng Liu[SUP] 1 [/SUP], Zhenle Qin[SUP] 2 [/SUP], Xiaobo Jia[SUP] 2 [/SUP], Jin Zhou[SUP] 2 [/SUP], Hongli Li[SUP] 2 [/SUP], Xiaohong Wang[SUP] 2 [/SUP], Yating Chen[SUP] 2 [/SUP], Junpeng Deng[SUP] 2 [/SUP], Zhicheng Jin[SUP] 3 [/SUP], Guofu Wang[SUP] 2 [/SUP]
Affiliations
- PMID: 37611522
- DOI: 10.1016/j.saa.2023.123275
The global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a great disaster to the economy and human society. Nowadays, SARS-CoV-2 is fading away from people's memory but it still exists around us. PCR plays an important role in detecting SARS-CoV-2 but it requires a long detecting time, equipped laboratory, and professional operators. In comparison with polymerase chain reaction (PCR), surface-enhanced Raman scattering (SERS) is a promising method for detecting SARS-CoV-2 due to its fast, easily operated, and high-sensitivity properties. In this study, the monolayer Ag nanoparticles (MAgNPs) covered with single-layer graphene (SLG) are applied as a SERS substrate. The angiotensin converting enzyme 2 (ACE2) is selected as a bio-probes that can specifically bind to the SARS-CoV-2 S protein. The SERS-based biosensor is formed by ACE2 functionalized SLG/MAgNPs and the LODs of detecting SARS-CoV-2 S protein in phosphate-buffered saline (PBS) and in pharyngeal swabs solution (PSS) are 0.1 fg mL[SUP]-1[/SUP] and 10 fg mL[SUP]-1[/SUP], respectively. This biosensor provides a way of directly detecting SARS-CoV-2 S protein with high sensitivity and specificity. It illustrates a practical potential in the rapid detection of the SARS-CoV-2 virus.
Keywords: ACE2 bio-probe; Biosensor; Monolayer Ag nanoparticles; SARS-CoV-2 S protein; Surface-enhanced Raman scattering (SERS).