tetano
Editor, Senior Moderator
Spectrochim Acta A Mol Biomol Spectrosc
. 2023 Sep 21;305:123445.
doi: 10.1016/j.saa.2023.123445. Online ahead of print. SERS-active immunoassay kit for SARS-CoV‑2 mediated by the cooperative chemical and electromagnetic effects of MXene modified with gold nanowires
Chucheng Wang[SUP] 1 [/SUP], Junshan Han[SUP] 1 [/SUP], Danni Xue[SUP] 1 [/SUP], Chenjie Gu[SUP] 1 [/SUP], Shuwen Zeng[SUP] 2 [/SUP], Junhui Jiang[SUP] 3 [/SUP], Tao Jiang[SUP] 4 [/SUP], Xing Li[SUP] 5 [/SUP], Kerong Wu[SUP] 6 [/SUP]
Affiliations
Surface-enhanced Raman scattering (SERS) technique with high sensitivity, reliable specificity, and rapid recognition ability exhibits attractive promise for the effective fast-monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, a novel SERS-active immunoassay kit for SARS-CoV-2 nucleocapsid (N) protein was prepared by in-situ growing gold (Au) nanowire forests (NFs) onto Ti[SUB]3[/SUB]C[SUB]2[/SUB]T[SUB]x[/SUB], which was then modified onto polymethyl methacrylate (PMMA) matrix and encapsulated into kit. It was noted that the Au nanowires with fibrous structures which vertically anchored on Ti[SUB]3[/SUB]C[SUB]2[/SUB]T[SUB]x[/SUB] served as perfect channels to promote photo-induced charge transfer. The synergistic action of electromagnetic and chemical effects resulted in an enhancement factor (EF) of 1.27 × 10[SUP]7[/SUP]. Furthermore, the unreliable fluctuation of the enhanced signal was eliminated by using the intrinsic Raman signal of the flexible PMMA platform, achieving an improved correlation coefficient (R[SUP]2[/SUP]) value from 0.950 to 0.990. Moreover, the as-designed immunoassay kit with both high sensitivity and remedied quantitative ability rendered by the Ti[SUB]3[/SUB]C[SUB]2[/SUB]T[SUB]x[/SUB]@Au NFs-PMMA composite exhibited a powerful performance in the practical detection of N-protein with concentration low to 5.0 × 10[SUP]-8[/SUP] mg/mL.
Keywords: Gold nanoflowers; Immunoassay kit; Internal standard; MXene; SARS-CoV-2.
. 2023 Sep 21;305:123445.
doi: 10.1016/j.saa.2023.123445. Online ahead of print. SERS-active immunoassay kit for SARS-CoV‑2 mediated by the cooperative chemical and electromagnetic effects of MXene modified with gold nanowires
Chucheng Wang[SUP] 1 [/SUP], Junshan Han[SUP] 1 [/SUP], Danni Xue[SUP] 1 [/SUP], Chenjie Gu[SUP] 1 [/SUP], Shuwen Zeng[SUP] 2 [/SUP], Junhui Jiang[SUP] 3 [/SUP], Tao Jiang[SUP] 4 [/SUP], Xing Li[SUP] 5 [/SUP], Kerong Wu[SUP] 6 [/SUP]
Affiliations
- PMID: 37757541
- DOI: 10.1016/j.saa.2023.123445
Surface-enhanced Raman scattering (SERS) technique with high sensitivity, reliable specificity, and rapid recognition ability exhibits attractive promise for the effective fast-monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, a novel SERS-active immunoassay kit for SARS-CoV-2 nucleocapsid (N) protein was prepared by in-situ growing gold (Au) nanowire forests (NFs) onto Ti[SUB]3[/SUB]C[SUB]2[/SUB]T[SUB]x[/SUB], which was then modified onto polymethyl methacrylate (PMMA) matrix and encapsulated into kit. It was noted that the Au nanowires with fibrous structures which vertically anchored on Ti[SUB]3[/SUB]C[SUB]2[/SUB]T[SUB]x[/SUB] served as perfect channels to promote photo-induced charge transfer. The synergistic action of electromagnetic and chemical effects resulted in an enhancement factor (EF) of 1.27 × 10[SUP]7[/SUP]. Furthermore, the unreliable fluctuation of the enhanced signal was eliminated by using the intrinsic Raman signal of the flexible PMMA platform, achieving an improved correlation coefficient (R[SUP]2[/SUP]) value from 0.950 to 0.990. Moreover, the as-designed immunoassay kit with both high sensitivity and remedied quantitative ability rendered by the Ti[SUB]3[/SUB]C[SUB]2[/SUB]T[SUB]x[/SUB]@Au NFs-PMMA composite exhibited a powerful performance in the practical detection of N-protein with concentration low to 5.0 × 10[SUP]-8[/SUP] mg/mL.
Keywords: Gold nanoflowers; Immunoassay kit; Internal standard; MXene; SARS-CoV-2.