tetano
Editor, Senior Moderator
STAR Protoc
. 2022 Feb 28;3(2):101238.
doi: 10.1016/j.xpro.2022.101238. eCollection 2022 Jun 17.
SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
Dan-Yun Lai[SUP] 1 [/SUP], He-Wei Jiang[SUP] 1 [/SUP], Yang Li[SUP] 2 [/SUP], Hai-Nan Zhang[SUP] 1 [/SUP], Sheng-Ce Tao[SUP] 1 [/SUP]
Affiliations
Abstract
The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either E. coli expression system or eukaryotic cell expression systems and obtained by affinity purification. The protocol includes microarray fabricating and sera profiling, which will be used to build an antibody response landscape for IgG and IgM. The protocol may help to facilitate a deeper understanding of immunity related to SARS-CoV-2. For complete details on the use and execution of this protocol, please refer to Li et al. (2021c).
Keywords: Biotechnology and bioengineering; Clinical Protocol; Health Sciences; Immunology; Protein Biochemistry; Protein expression and purification; Proteomics.
. 2022 Feb 28;3(2):101238.
doi: 10.1016/j.xpro.2022.101238. eCollection 2022 Jun 17.
SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
Dan-Yun Lai[SUP] 1 [/SUP], He-Wei Jiang[SUP] 1 [/SUP], Yang Li[SUP] 2 [/SUP], Hai-Nan Zhang[SUP] 1 [/SUP], Sheng-Ce Tao[SUP] 1 [/SUP]
Affiliations
- PMID: 35310073
- PMCID: PMC8882418
- DOI: 10.1016/j.xpro.2022.101238
Abstract
The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either E. coli expression system or eukaryotic cell expression systems and obtained by affinity purification. The protocol includes microarray fabricating and sera profiling, which will be used to build an antibody response landscape for IgG and IgM. The protocol may help to facilitate a deeper understanding of immunity related to SARS-CoV-2. For complete details on the use and execution of this protocol, please refer to Li et al. (2021c).
Keywords: Biotechnology and bioengineering; Clinical Protocol; Health Sciences; Immunology; Protein Biochemistry; Protein expression and purification; Proteomics.