tetano
Editor, Senior Moderator
Cold Spring Harb Perspect Med. 2019 Dec 23. pii: a038398. doi: 10.1101/cshperspect.a038398. [Epub ahead of print] [h=1]Structure and Function of the Influenza Virus Transcription and Replication Machinery.[/h]
Fodor E[SUP]1[/SUP], Te Velthuis AJW[SUP]2[/SUP].
[h=3]Author information[/h] 1 Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. 2 Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 2QQ, United Kingdom.
[h=3]Abstract[/h] Transcription and replication of the influenza virus RNA genome is catalyzed by the viral heterotrimeric RNA-dependent RNA polymerase in the context of viral ribonucleoprotein (vRNP) complexes. Atomic resolution structures of the viral RNA synthesis machinery have offered insights into the initiation mechanisms of viral transcription and genome replication, and the interaction of the viral RNA polymerase with host RNA polymerase II, which is required for the initiation of viral transcription. Replication of the viral RNA genome by the viral RNA polymerase depends on host ANP32A, and host-specific sequence differences in ANP32A underlie the poor activity of avian influenza virus polymerases in mammalian cells. A failure to faithfully copy the viral genome segments can lead to the production of aberrant viral RNA products, such as defective interfering (DI) RNAs and mini viral RNAs (mvRNAs). Both aberrant RNA types have been implicated in innate immune responses against influenza virus infection. This review discusses recent insights into the structure-function relationship of the viral RNA polymerase and its role in determining host range and virulence.
Copyright ? 2019 Cold Spring Harbor Laboratory Press; all rights reserved.
PMID: 31871230 DOI: 10.1101/cshperspect.a038398
Fodor E[SUP]1[/SUP], Te Velthuis AJW[SUP]2[/SUP].
[h=3]Author information[/h] 1 Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. 2 Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 2QQ, United Kingdom.
[h=3]Abstract[/h] Transcription and replication of the influenza virus RNA genome is catalyzed by the viral heterotrimeric RNA-dependent RNA polymerase in the context of viral ribonucleoprotein (vRNP) complexes. Atomic resolution structures of the viral RNA synthesis machinery have offered insights into the initiation mechanisms of viral transcription and genome replication, and the interaction of the viral RNA polymerase with host RNA polymerase II, which is required for the initiation of viral transcription. Replication of the viral RNA genome by the viral RNA polymerase depends on host ANP32A, and host-specific sequence differences in ANP32A underlie the poor activity of avian influenza virus polymerases in mammalian cells. A failure to faithfully copy the viral genome segments can lead to the production of aberrant viral RNA products, such as defective interfering (DI) RNAs and mini viral RNAs (mvRNAs). Both aberrant RNA types have been implicated in innate immune responses against influenza virus infection. This review discusses recent insights into the structure-function relationship of the viral RNA polymerase and its role in determining host range and virulence.
Copyright ? 2019 Cold Spring Harbor Laboratory Press; all rights reserved.
PMID: 31871230 DOI: 10.1101/cshperspect.a038398