tetano
Editor, Senior Moderator
Virology
Rapid Communication
Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice
Huachen Zhua, b, Jia Wanga, b, Pui Wanga, b, Wenjun Songa, b, Zuoyi Zhenga, Rirong Chena, Kunyuan Guoa, Taixing Zhanga, Joseph S.M. Peirisb, Honglin Chena, b, Corresponding Author Contact Information, E-mail The Corresponding Author and Yi Guana, b, Corresponding Author Contact Information, E-mail The Corresponding Author
a International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong 515031, PR China
b State Key Laboratory of Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, PR China
Received 26 November 2009;
revised 11 January 2010;
accepted 18 February 2010.
Available online 19 March 2010.
Abstract
A lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6 months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus.
http://www.sciencedirect.com/scienc...serid=10&md5=2c7e7fa50602d2e9daef8604bf53fe09
Rapid Communication
Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice
Huachen Zhua, b, Jia Wanga, b, Pui Wanga, b, Wenjun Songa, b, Zuoyi Zhenga, Rirong Chena, Kunyuan Guoa, Taixing Zhanga, Joseph S.M. Peirisb, Honglin Chena, b, Corresponding Author Contact Information, E-mail The Corresponding Author and Yi Guana, b, Corresponding Author Contact Information, E-mail The Corresponding Author
a International Institute of Infection and Immunity, Shantou University Medical College, Shantou, Guangdong 515031, PR China
b State Key Laboratory of Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, PR China
Received 26 November 2009;
revised 11 January 2010;
accepted 18 February 2010.
Available online 19 March 2010.
Abstract
A lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6 months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus.
http://www.sciencedirect.com/scienc...serid=10&md5=2c7e7fa50602d2e9daef8604bf53fe09