tetano
Editor, Senior Moderator
Biochim Biophys Acta. 2015 Jun 16. pii: S0005-2736(15)00195-9. doi: 10.1016/j.bbamem.2015.06.015. [Epub ahead of print]
[h=1]Super short membrane-active lipopeptides inhibiting the entry of influenza A virus.[/h] Wu W[SUP]1[/SUP], Wang J[SUP]1[/SUP], Lin D[SUP]1[/SUP], Chen L[SUP]1[/SUP], Xie X[SUP]1[/SUP], Shen X[SUP]1[/SUP], Yang Q[SUP]1[/SUP], Wu Q[SUP]1[/SUP], Yang J[SUP]1[/SUP], He J[SUP]2[/SUP], Liu S[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses (IAV) are significant pathogens that result in millions of human infections and impose a substantial health and economic burdens worldwide. Due to the limited anti-influenza A therapeutics available and the emergence of drug resistant viral strains, it is imperative to develop potent anti-IAV agents with different mode of action. In this study, by applying a pseudovirus based screening approach, two super short membrane-active lipopeptides of C12-KKWK and C12-OOWO were identified as effective anti-IAV agents with IC[SUB]50[/SUB] value of 7.30?1.57 and 8.48?0.74 mg/L against A/Puerto Rico/8/34 strain, and 6.14?1.45 and 7.22?0.67 mg/L against A/Aichi/2/68 strain, respectively. The mechanism study indicated that the anti-IAV activity of these peptides would result from the inhibition of virus entry by interacting with HA2 subunit of hemagglutinin (HA). Thus, these peptides may have potentials as lead peptides for the development of new anti-IAV therapeutics to block the entry of virus into host cells.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Influenza A virus; fusion inhibition; membrane fusion; short lipopeptide; virus entry inhibitor; virus-cell fusion
PMID: 26092189 [PubMed - as supplied by publisher]
[h=1]Super short membrane-active lipopeptides inhibiting the entry of influenza A virus.[/h] Wu W[SUP]1[/SUP], Wang J[SUP]1[/SUP], Lin D[SUP]1[/SUP], Chen L[SUP]1[/SUP], Xie X[SUP]1[/SUP], Shen X[SUP]1[/SUP], Yang Q[SUP]1[/SUP], Wu Q[SUP]1[/SUP], Yang J[SUP]1[/SUP], He J[SUP]2[/SUP], Liu S[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses (IAV) are significant pathogens that result in millions of human infections and impose a substantial health and economic burdens worldwide. Due to the limited anti-influenza A therapeutics available and the emergence of drug resistant viral strains, it is imperative to develop potent anti-IAV agents with different mode of action. In this study, by applying a pseudovirus based screening approach, two super short membrane-active lipopeptides of C12-KKWK and C12-OOWO were identified as effective anti-IAV agents with IC[SUB]50[/SUB] value of 7.30?1.57 and 8.48?0.74 mg/L against A/Puerto Rico/8/34 strain, and 6.14?1.45 and 7.22?0.67 mg/L against A/Aichi/2/68 strain, respectively. The mechanism study indicated that the anti-IAV activity of these peptides would result from the inhibition of virus entry by interacting with HA2 subunit of hemagglutinin (HA). Thus, these peptides may have potentials as lead peptides for the development of new anti-IAV therapeutics to block the entry of virus into host cells.
Copyright ? 2015. Published by Elsevier B.V.
[h=4]KEYWORDS:[/h] Influenza A virus; fusion inhibition; membrane fusion; short lipopeptide; virus entry inhibitor; virus-cell fusion
PMID: 26092189 [PubMed - as supplied by publisher]