tetano
Editor, Senior Moderator
Equine Vet J. 2013 May 8. doi: 10.1111/evj.12100. [Epub ahead of print]
Surveillance of equine influenza viruses through the RESPE network in France from November 2005 to October 2010.
Legrand LJ, Pitel PH, Marcillaud-Pitel CJ, Cullinane AA, Courouc? AM, Fortier GD, Freymuth FL, Pronost SL.
Source
Frank Duncombe Laboratory; 1 Route de Rosel, 14053 Caen Cedex 4, France; EA 4655 U2RM -University of Caen Basse-Normandy, 14000 Caen, France.
Abstract
REASONS FOR PERFORMING THE STUDY:
RESPE (French epidemiological network for equine diseases) is a network for epidemio-surveillance of major equine diseases based around sentry veterinarians in France.
OBJECTIVE:
The aim of this study was to evaluate the contribution of RESPE to efficient surveillance of EIV in France.
STUDY DESIGN:
Retrospective cross-sectional study METHODS: From November 2005 to October 2010, epidemiological and phylogenetic studies were performed on 1426 nasopharyngeal swabs received at the Frank Duncombe Laboratory. Detection was performed by real-time RT-PCR using original primers and probes designed in the matrix protein gene. Phylogenetic analysis was carried out on the HA1 part of haemagglutinin gene amplified from 47 positive-testing samples. Epidemiological information was provided with the majority of samples submitted through RESPE.
RESULTS:
Of the 920 samples submitted by RESPE-associated veterinarians, 121 (13.1%) from 42 premises were positive for EIV, compared to 26 (5.1%) of the 607 samples received from non RESPE-associated veterinarians. The most extensive outbreak was observed between February and May 2009, affecting 70 horses on 23 premises, 15 of these premises were managed by RESPE-associated veterinarians. All strains belonged to the American lineage, Florida sublineage, Clade 1 and Clade 2. Clade 1 was identified only during the Grosbois episode.
CONCLUSION:
RESPE improved detection of EIV in France, enabled characterisation of the virus strains, yielded valuable information relating to the epidemiology of the disease and identified vaccine breakdown.
POTENTIAL RELEVANCE:
Implementation of a similar surveillance network in other countries may reduce the economic losses associated with outbreaks of EIV.
This article is protected by copyright. All rights reserved.
PMID:
23662725
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23662725
Surveillance of equine influenza viruses through the RESPE network in France from November 2005 to October 2010.
Legrand LJ, Pitel PH, Marcillaud-Pitel CJ, Cullinane AA, Courouc? AM, Fortier GD, Freymuth FL, Pronost SL.
Source
Frank Duncombe Laboratory; 1 Route de Rosel, 14053 Caen Cedex 4, France; EA 4655 U2RM -University of Caen Basse-Normandy, 14000 Caen, France.
Abstract
REASONS FOR PERFORMING THE STUDY:
RESPE (French epidemiological network for equine diseases) is a network for epidemio-surveillance of major equine diseases based around sentry veterinarians in France.
OBJECTIVE:
The aim of this study was to evaluate the contribution of RESPE to efficient surveillance of EIV in France.
STUDY DESIGN:
Retrospective cross-sectional study METHODS: From November 2005 to October 2010, epidemiological and phylogenetic studies were performed on 1426 nasopharyngeal swabs received at the Frank Duncombe Laboratory. Detection was performed by real-time RT-PCR using original primers and probes designed in the matrix protein gene. Phylogenetic analysis was carried out on the HA1 part of haemagglutinin gene amplified from 47 positive-testing samples. Epidemiological information was provided with the majority of samples submitted through RESPE.
RESULTS:
Of the 920 samples submitted by RESPE-associated veterinarians, 121 (13.1%) from 42 premises were positive for EIV, compared to 26 (5.1%) of the 607 samples received from non RESPE-associated veterinarians. The most extensive outbreak was observed between February and May 2009, affecting 70 horses on 23 premises, 15 of these premises were managed by RESPE-associated veterinarians. All strains belonged to the American lineage, Florida sublineage, Clade 1 and Clade 2. Clade 1 was identified only during the Grosbois episode.
CONCLUSION:
RESPE improved detection of EIV in France, enabled characterisation of the virus strains, yielded valuable information relating to the epidemiology of the disease and identified vaccine breakdown.
POTENTIAL RELEVANCE:
Implementation of a similar surveillance network in other countries may reduce the economic losses associated with outbreaks of EIV.
This article is protected by copyright. All rights reserved.
PMID:
23662725
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23662725