tetano
Editor, Senior Moderator
Int J Infect Dis. 2017 Jun 15. pii: S1201-9712(17)30165-0. doi: 10.1016/j.ijid.2017.06.011. [Epub ahead of print]
[h=1]Surveillance of upper respiratory infections by using newly multiplex PCR assay compared to conventional methods during influenza season in Taiwan.[/h] Chiu SC[SUP]1[/SUP], Lin YC[SUP]2[/SUP], Wang HC[SUP]3[/SUP], Hsu JJ[SUP]1[/SUP], Yeh TK[SUP]1[/SUP], Liu HF[SUP]4[/SUP], Lin JH[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVES:[/h] To improve the diagnosis of laboratory surveillance, we conducted influenza-like illness (ILI) surveillance by using newly multiplex PCR Assay (FilmArray) compared to conventional methods during winter period in Taiwan.
[h=4]METHODS:[/h] Throat swabs from ILI patients presenting to physicians in sentinel practices were collected during 2016-2017 influenza season.
[h=4]RESULTS:[/h] A total of 52 samples were tested positive by FilmArray-Respiratory Panel, 40% of them were influenza A virus, subtype H3N2 viruses were the major epidemic strains. However, nearly 60% of ILI cases seen at sentinel sites were caused by non-influenza pathogens. Results from FilmArray and cell culture were identical to each other, and were higher sensitive than rapid influenza diagnostic test. Genetic analyses indicated new influenza A (H3N2) variants were observed which belong to a novel subclade 3C.2a2.
[h=4]CONCLUSIONS:[/h] The FilmArray facilitates urgent testing and laboratory surveillance for common viral and bacterial respiratory pathogens. Our study demonstrates the use of more sensitive methods with clinical samples is a feasible application in real world and has led to increased diagnostic rate of viral infection and to improve patient outcomes, especially a reduction on the overuse of antibiotics and antivirals.
Copyright ? 2017. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Influenza virus; Taiwan; URI; multiplex PCR
PMID: 28625839 DOI: 10.1016/j.ijid.2017.06.011
[h=1]Surveillance of upper respiratory infections by using newly multiplex PCR assay compared to conventional methods during influenza season in Taiwan.[/h] Chiu SC[SUP]1[/SUP], Lin YC[SUP]2[/SUP], Wang HC[SUP]3[/SUP], Hsu JJ[SUP]1[/SUP], Yeh TK[SUP]1[/SUP], Liu HF[SUP]4[/SUP], Lin JH[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVES:[/h] To improve the diagnosis of laboratory surveillance, we conducted influenza-like illness (ILI) surveillance by using newly multiplex PCR Assay (FilmArray) compared to conventional methods during winter period in Taiwan.
[h=4]METHODS:[/h] Throat swabs from ILI patients presenting to physicians in sentinel practices were collected during 2016-2017 influenza season.
[h=4]RESULTS:[/h] A total of 52 samples were tested positive by FilmArray-Respiratory Panel, 40% of them were influenza A virus, subtype H3N2 viruses were the major epidemic strains. However, nearly 60% of ILI cases seen at sentinel sites were caused by non-influenza pathogens. Results from FilmArray and cell culture were identical to each other, and were higher sensitive than rapid influenza diagnostic test. Genetic analyses indicated new influenza A (H3N2) variants were observed which belong to a novel subclade 3C.2a2.
[h=4]CONCLUSIONS:[/h] The FilmArray facilitates urgent testing and laboratory surveillance for common viral and bacterial respiratory pathogens. Our study demonstrates the use of more sensitive methods with clinical samples is a feasible application in real world and has led to increased diagnostic rate of viral infection and to improve patient outcomes, especially a reduction on the overuse of antibiotics and antivirals.
Copyright ? 2017. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Influenza virus; Taiwan; URI; multiplex PCR
PMID: 28625839 DOI: 10.1016/j.ijid.2017.06.011