tetano
Editor, Senior Moderator
Zhonghua Yu Fang Yi Xue Za Zhi. 2015 Jun;49(6):481-484.
[h=1][Susceptibility of human influenza A (H3N2) viruses to neuraminidase inhibitors isolated during 2011-2012 in China].[/h] [Article in Chinese]
Huang W[SUP]1[/SUP], Tan M[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Cheng Y[SUP]1[/SUP], Li X[SUP]1[/SUP], Guo J[SUP]1[/SUP], Wei H[SUP]1[/SUP], Xiao N[SUP]1[/SUP], Wang Z[SUP]1[/SUP], Wang D[SUP]2[/SUP], Shu Y[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] To analyze the susceptibility of influenza A (H3N2) viruses to neuraminidase inhibitors during 2011-2012 in Mainland China.
[h=4]METHODS:[/h] All the tested viruses were obtained from the Chinese National Influenza Surveillance Network, which covers 31 provinces in mainland China, including 408 network laboratories and 554 sentinel hospitals. In total 1 903 viruses were selected with isolation date from January 1, 2011 to December 31, 2012 in Mainland China, among these viruses, 721 were confirmed to be influenza A (H3N2) virus by Chinese National Influenza Center and tested for the susceptibility to oseltamivir and zanamivir using chemiluminescence-based assay. The neuraminidase inhibitor sensitive reference virus A/Washington/01/2007 (119E) and oseltamivir resistant virus A/Texas/12/2007 (E119V) were used as control in this study. The t -test was used to compare the difference of NAI susceptibility of viruses isolated from different years.
[h=4]RESULTS:[/h] The half maximal inhibitory concentration (IC[SUB]50[/SUB]) of A/Washington/01/2007 for oseltamivir and zanamivir was (0.10 ? 0.02) and (0.30 ? 0.05) nmol/L, respectively. The IC[SUB]50[/SUB] of A/Texas/12/2007 for oseltamivir and zanamivir was (4.27 ? 1.60) and (0.20 ? 0.03) nmol/L, respectively. Among the 721 influenza A (H3N2) viruses, 132 influenza A (H3N2) viruses were isolated in 2011 and 589 influenza A (H3N2) viruses were isolated in 2012. The IC[SUB]50[/SUB] for oseltamivir ranged from 0.04 to 0.62 nmol/L for viruses isolated in 2011 and ranged from 0.02 to 0.95 nmol/L for viruses in 2012, and the IC[SUB]50[/SUB] of all the viruses tested was within 10-fold IC[SUB]50[/SUB] (1.0 nmol/L) of the neuraminidase inhibitor sensitive reference virus A/Washington/01/2007. The IC[SUB]50[/SUB] of zanamivir ranged from 0.12 to 0.80 nmol/L for viruses in 2011 and ranged from 0.04 to 0.72 nmol/L for viruses in 2012, and was within 10-fold IC[SUB]50[/SUB] (3.0 nmol/L) of the neuraminidase inhibitor sensitive reference virus A/Washington/01/2007.
[h=4]CONCLUSION:[/h] The influenza A(H3N2) viruses isolated during 2011-2012 in Mainland China were tested to be sensitive to oseltamivir and zanamivir.
PMID: 26310330 [PubMed - as supplied by publisher]
[h=1][Susceptibility of human influenza A (H3N2) viruses to neuraminidase inhibitors isolated during 2011-2012 in China].[/h] [Article in Chinese]
Huang W[SUP]1[/SUP], Tan M[SUP]1[/SUP], Zhao X[SUP]1[/SUP], Cheng Y[SUP]1[/SUP], Li X[SUP]1[/SUP], Guo J[SUP]1[/SUP], Wei H[SUP]1[/SUP], Xiao N[SUP]1[/SUP], Wang Z[SUP]1[/SUP], Wang D[SUP]2[/SUP], Shu Y[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]OBJECTIVE:[/h] To analyze the susceptibility of influenza A (H3N2) viruses to neuraminidase inhibitors during 2011-2012 in Mainland China.
[h=4]METHODS:[/h] All the tested viruses were obtained from the Chinese National Influenza Surveillance Network, which covers 31 provinces in mainland China, including 408 network laboratories and 554 sentinel hospitals. In total 1 903 viruses were selected with isolation date from January 1, 2011 to December 31, 2012 in Mainland China, among these viruses, 721 were confirmed to be influenza A (H3N2) virus by Chinese National Influenza Center and tested for the susceptibility to oseltamivir and zanamivir using chemiluminescence-based assay. The neuraminidase inhibitor sensitive reference virus A/Washington/01/2007 (119E) and oseltamivir resistant virus A/Texas/12/2007 (E119V) were used as control in this study. The t -test was used to compare the difference of NAI susceptibility of viruses isolated from different years.
[h=4]RESULTS:[/h] The half maximal inhibitory concentration (IC[SUB]50[/SUB]) of A/Washington/01/2007 for oseltamivir and zanamivir was (0.10 ? 0.02) and (0.30 ? 0.05) nmol/L, respectively. The IC[SUB]50[/SUB] of A/Texas/12/2007 for oseltamivir and zanamivir was (4.27 ? 1.60) and (0.20 ? 0.03) nmol/L, respectively. Among the 721 influenza A (H3N2) viruses, 132 influenza A (H3N2) viruses were isolated in 2011 and 589 influenza A (H3N2) viruses were isolated in 2012. The IC[SUB]50[/SUB] for oseltamivir ranged from 0.04 to 0.62 nmol/L for viruses isolated in 2011 and ranged from 0.02 to 0.95 nmol/L for viruses in 2012, and the IC[SUB]50[/SUB] of all the viruses tested was within 10-fold IC[SUB]50[/SUB] (1.0 nmol/L) of the neuraminidase inhibitor sensitive reference virus A/Washington/01/2007. The IC[SUB]50[/SUB] of zanamivir ranged from 0.12 to 0.80 nmol/L for viruses in 2011 and ranged from 0.04 to 0.72 nmol/L for viruses in 2012, and was within 10-fold IC[SUB]50[/SUB] (3.0 nmol/L) of the neuraminidase inhibitor sensitive reference virus A/Washington/01/2007.
[h=4]CONCLUSION:[/h] The influenza A(H3N2) viruses isolated during 2011-2012 in Mainland China were tested to be sensitive to oseltamivir and zanamivir.
PMID: 26310330 [PubMed - as supplied by publisher]