Mary Wilson
Well-known member
Pages 2278-2288 | Received 17 Aug 2020, Accepted 28 Sep 2020, Accepted author version posted online: 02 Oct 2020, Published online:20 Oct 2020
https://doi.org/10.1080/22221751.2020.1831405
David A. Meekins , Igor Morozov , Jessie D. Trujillo , Natasha N. Gaudreault , Dashzeveg Bold , Mariano Carossino , Bianca L. Artiaga , Sabarish V. Indran , Taeyong Kwon , Velmurugan Balaraman , Daniel W. Madden , Heinz Feldmann , Jamie Henningson , Wenjun Ma , Udeni B. R. Balasuriya & Juergen A. Richt
ABSTRACT
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed na?ve sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.
https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1831405
https://doi.org/10.1080/22221751.2020.1831405
David A. Meekins , Igor Morozov , Jessie D. Trujillo , Natasha N. Gaudreault , Dashzeveg Bold , Mariano Carossino , Bianca L. Artiaga , Sabarish V. Indran , Taeyong Kwon , Velmurugan Balaraman , Daniel W. Madden , Heinz Feldmann , Jamie Henningson , Wenjun Ma , Udeni B. R. Balasuriya & Juergen A. Richt
ABSTRACT
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed na?ve sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.
https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1831405