tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2017 Oct 20. doi: 10.1111/irv.12513. [Epub ahead of print]
[h=1]T cell epitope Content Comparison (EpiCC) of swine H1 influenza A virus hemagglutinin.[/h] Guti?rrez AH[SUP]1[/SUP], Rapp-Gabrielson V[SUP]2[/SUP], Terry FE[SUP]3[/SUP], Loving C[SUP]4[/SUP], Moise L[SUP]1,[/SUP][SUP]3[/SUP], Martin WD[SUP]3[/SUP], De Groot AS[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Predicting vaccine efficacy against emerging pathogen strains is a significant problem in human and animal vaccine design. T cell epitope cross-conservation may play an important role in cross-strain vaccine efficacy. While influenza A virus (IAV) hemagglutination inhibition (HI) antibody titers are widely used to predict protective efficacy of one IAV vaccine against new strains, no similar correlate of protection has been identified for T cell epitopes.
[h=4]OBJECTIVE:[/h] We developed a computational method (EpiCC) that facilitates pairwise comparison of protein sequences based on an immunological property - T cell epitope content - rather than sequence identity, and evaluated its ability to classify swine IAV strain relatedness to estimate cross-protective potential of a vaccine strain for circulating viruses.
[h=4]METHODS:[/h] T cell epitope relatedness scores were assessed for 23 IAV HA sequences representing the major H1 swine IAV phylo-clusters circulating in North American swine and HA sequences in a commercial inactivated vaccine (FluSure XP[SUP]?[/SUP] ). Scores were compared to experimental data from previous efficacy studies.
[h=4]RESULTS:[/h] Higher EpiCC scores were associated with greater protection by the vaccine against strains for 23 field IAV strain-vaccine comparisons. A threshold for EpiCC relatedness associated with full or partial protection in the absence of cross-reactive HI antibodies was identified. EpiCC scores for field strains for which FluSure protective efficacy is not yet available, were also calculated.
[h=4]CONCLUSION:[/h] EpiCC thresholds can be evaluated for predictive accuracy of protection in future efficacy studies. EpiCC may also complement HI cross-reactivity and phylogeny for selection of influenza strains in vaccine development. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] IAV ; SLA ; Computational immunology; Hemagglutinin; T cell epitope content comparison; T cell epitope prediction; swine influenza H1 viruses; vaccine efficacy
PMID: 29054116 DOI: 10.1111/irv.12513
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[h=1]T cell epitope Content Comparison (EpiCC) of swine H1 influenza A virus hemagglutinin.[/h] Guti?rrez AH[SUP]1[/SUP], Rapp-Gabrielson V[SUP]2[/SUP], Terry FE[SUP]3[/SUP], Loving C[SUP]4[/SUP], Moise L[SUP]1,[/SUP][SUP]3[/SUP], Martin WD[SUP]3[/SUP], De Groot AS[SUP]1,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Predicting vaccine efficacy against emerging pathogen strains is a significant problem in human and animal vaccine design. T cell epitope cross-conservation may play an important role in cross-strain vaccine efficacy. While influenza A virus (IAV) hemagglutination inhibition (HI) antibody titers are widely used to predict protective efficacy of one IAV vaccine against new strains, no similar correlate of protection has been identified for T cell epitopes.
[h=4]OBJECTIVE:[/h] We developed a computational method (EpiCC) that facilitates pairwise comparison of protein sequences based on an immunological property - T cell epitope content - rather than sequence identity, and evaluated its ability to classify swine IAV strain relatedness to estimate cross-protective potential of a vaccine strain for circulating viruses.
[h=4]METHODS:[/h] T cell epitope relatedness scores were assessed for 23 IAV HA sequences representing the major H1 swine IAV phylo-clusters circulating in North American swine and HA sequences in a commercial inactivated vaccine (FluSure XP[SUP]?[/SUP] ). Scores were compared to experimental data from previous efficacy studies.
[h=4]RESULTS:[/h] Higher EpiCC scores were associated with greater protection by the vaccine against strains for 23 field IAV strain-vaccine comparisons. A threshold for EpiCC relatedness associated with full or partial protection in the absence of cross-reactive HI antibodies was identified. EpiCC scores for field strains for which FluSure protective efficacy is not yet available, were also calculated.
[h=4]CONCLUSION:[/h] EpiCC thresholds can be evaluated for predictive accuracy of protection in future efficacy studies. EpiCC may also complement HI cross-reactivity and phylogeny for selection of influenza strains in vaccine development. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] IAV ; SLA ; Computational immunology; Hemagglutinin; T cell epitope content comparison; T cell epitope prediction; swine influenza H1 viruses; vaccine efficacy
PMID: 29054116 DOI: 10.1111/irv.12513
Free full text