tetano
Editor, Senior Moderator
Talanta
. 2023 May 5;260:124614.
doi: 10.1016/j.talanta.2023.124614. Online ahead of print. Bifunctional Au@Pt/Au nanoparticles as electrochemiluminescence signaling probes for SARS-CoV-2 detection
Ana M Villa-Manso[SUP] 1 [/SUP], Tamara Guerrero-Esteban[SUP] 1 [/SUP], Félix Pariente[SUP] 2 [/SUP], Celia Toyos-Rodríguez[SUP] 3 [/SUP], Alfredo de la Escosura-Muñiz[SUP] 3 [/SUP], Mónica Revenga-Parra[SUP] 4 [/SUP], Cristina Gutiérrez-Sánchez[SUP] 5 [/SUP], Encarnación Lorenzo[SUP] 6 [/SUP]
Affiliations
A novel immunosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) for the sensitive determination of N protein of the SARS-CoV-2 coronavirus is described. For this purpose, bifunctional core@shell nanoparticles composed of a Pt-coated Au core and finally decorated with small Au inlays (Au@Pt/Au NPs) have been synthesized to act as ECL acceptor, using [Ru (bpy)[SUB]3[/SUB]][SUP]2+[/SUP] as ECL donor. These nanoparticles are efficient signaling probes in the immunosensor developed. The proposed ECL-RET immunosensor has a wide linear response to the concentration of N protein of the SARS-CoV-2 coronavirus with a detection limit of 1.27 pg/mL. Moreover, it has a high stability and shows no response to other proteins related to different virus. The immunosensor has achieved the quantification of N protein of the SARS-CoV-2 coronavirus in saliva samples. Results are consistent with those provided by a commercial colorimetric ELISA kit. Therefore, the developed immunosensor provides a feasible and reliable tool for early and effective detection of the virus to protect the population.
Keywords: COVID-19; Electrochemiluminescence resonance energy transfer; Immunosensor; Metal nanoparticles; N protein; SARS-CoV-2.
. 2023 May 5;260:124614.
doi: 10.1016/j.talanta.2023.124614. Online ahead of print. Bifunctional Au@Pt/Au nanoparticles as electrochemiluminescence signaling probes for SARS-CoV-2 detection
Ana M Villa-Manso[SUP] 1 [/SUP], Tamara Guerrero-Esteban[SUP] 1 [/SUP], Félix Pariente[SUP] 2 [/SUP], Celia Toyos-Rodríguez[SUP] 3 [/SUP], Alfredo de la Escosura-Muñiz[SUP] 3 [/SUP], Mónica Revenga-Parra[SUP] 4 [/SUP], Cristina Gutiérrez-Sánchez[SUP] 5 [/SUP], Encarnación Lorenzo[SUP] 6 [/SUP]
Affiliations
- PMID: 37163926
- DOI: 10.1016/j.talanta.2023.124614
A novel immunosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) for the sensitive determination of N protein of the SARS-CoV-2 coronavirus is described. For this purpose, bifunctional core@shell nanoparticles composed of a Pt-coated Au core and finally decorated with small Au inlays (Au@Pt/Au NPs) have been synthesized to act as ECL acceptor, using [Ru (bpy)[SUB]3[/SUB]][SUP]2+[/SUP] as ECL donor. These nanoparticles are efficient signaling probes in the immunosensor developed. The proposed ECL-RET immunosensor has a wide linear response to the concentration of N protein of the SARS-CoV-2 coronavirus with a detection limit of 1.27 pg/mL. Moreover, it has a high stability and shows no response to other proteins related to different virus. The immunosensor has achieved the quantification of N protein of the SARS-CoV-2 coronavirus in saliva samples. Results are consistent with those provided by a commercial colorimetric ELISA kit. Therefore, the developed immunosensor provides a feasible and reliable tool for early and effective detection of the virus to protect the population.
Keywords: COVID-19; Electrochemiluminescence resonance energy transfer; Immunosensor; Metal nanoparticles; N protein; SARS-CoV-2.