tetano
Editor, Senior Moderator
Talanta
. 2023 Oct 13;268(Pt 1):125279.
doi: 10.1016/j.talanta.2023.125279. Online ahead of print. Detection of antibodies against SARS-CoV-2 Spike protein by screen-printed carbon electrodes modified by colloidal gold nanoparticles
Ausra Baradoke[SUP] 1 [/SUP], Ainis Jarusaitis[SUP] 2 [/SUP], Viktorija Reinikovaite[SUP] 2 [/SUP], Ali Jafarov[SUP] 3 [/SUP], Alexandra Elsakova[SUP] 4 [/SUP], Marius Franckevicius[SUP] 1 [/SUP], Martynas Skapas[SUP] 1 [/SUP], Rimantas Slibinskas[SUP] 5 [/SUP], Maryia Drobysh[SUP] 2 [/SUP], Viktorija Liustrovaite[SUP] 2 [/SUP], Arunas Ramanavicius[SUP] 6 [/SUP]
Affiliations
In this work, electrochemical bioanalytical systems for the determination of antibodies against the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike protein (anti-rS) is reported. Environmentally friendly chemicals were applied in the synthesis of gold nanoparticles (AuNPs). The AuNPs were integrated onto the screen-printed carbon electrodes (SPE), and the biological recognition part was based on recombinant SARS-CoV-2 Spike protein (rS), which during the immobilization was cross-linked by glutaraldehyde. Immobilized rS protein based biological recognition part enabled selective recognition of anti-rS antibodies. The current flux of AuNPs reduction (at +200 mV) in a pure phosphate buffer (PB) was employed as the transduction signal. It has been reported that the formation of anti-rS layers on the surface of AuNPs delays the electrode response time (t[SUB]s[/SUB]), tracked at the current flux density values of 80 μA cm[SUP]-2[/SUP]. Using the AuNP-modified SPE, we demonstrated a rapid anti-rS detection within a detection limit of 2 ng mL[SUP]-1[/SUP] (0.125 binding antibody units mL[SUP]-1[/SUP], 17 pM). This system can be applied to track the response of immune system towards SARS-CoV-2 infection and monitoring of Coronavirus Disease 2019 (COVID-19).
Keywords: COVID-19; Chronoamperometry; Cyclic voltammetry; Electrochemistry; Gold nanoparticles (AuNPs); Green synthesis; SARS-CoV-2.
. 2023 Oct 13;268(Pt 1):125279.
doi: 10.1016/j.talanta.2023.125279. Online ahead of print. Detection of antibodies against SARS-CoV-2 Spike protein by screen-printed carbon electrodes modified by colloidal gold nanoparticles
Ausra Baradoke[SUP] 1 [/SUP], Ainis Jarusaitis[SUP] 2 [/SUP], Viktorija Reinikovaite[SUP] 2 [/SUP], Ali Jafarov[SUP] 3 [/SUP], Alexandra Elsakova[SUP] 4 [/SUP], Marius Franckevicius[SUP] 1 [/SUP], Martynas Skapas[SUP] 1 [/SUP], Rimantas Slibinskas[SUP] 5 [/SUP], Maryia Drobysh[SUP] 2 [/SUP], Viktorija Liustrovaite[SUP] 2 [/SUP], Arunas Ramanavicius[SUP] 6 [/SUP]
Affiliations
- PMID: 37857108
- DOI: 10.1016/j.talanta.2023.125279
In this work, electrochemical bioanalytical systems for the determination of antibodies against the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Spike protein (anti-rS) is reported. Environmentally friendly chemicals were applied in the synthesis of gold nanoparticles (AuNPs). The AuNPs were integrated onto the screen-printed carbon electrodes (SPE), and the biological recognition part was based on recombinant SARS-CoV-2 Spike protein (rS), which during the immobilization was cross-linked by glutaraldehyde. Immobilized rS protein based biological recognition part enabled selective recognition of anti-rS antibodies. The current flux of AuNPs reduction (at +200 mV) in a pure phosphate buffer (PB) was employed as the transduction signal. It has been reported that the formation of anti-rS layers on the surface of AuNPs delays the electrode response time (t[SUB]s[/SUB]), tracked at the current flux density values of 80 μA cm[SUP]-2[/SUP]. Using the AuNP-modified SPE, we demonstrated a rapid anti-rS detection within a detection limit of 2 ng mL[SUP]-1[/SUP] (0.125 binding antibody units mL[SUP]-1[/SUP], 17 pM). This system can be applied to track the response of immune system towards SARS-CoV-2 infection and monitoring of Coronavirus Disease 2019 (COVID-19).
Keywords: COVID-19; Chronoamperometry; Cyclic voltammetry; Electrochemistry; Gold nanoparticles (AuNPs); Green synthesis; SARS-CoV-2.