tetano
Editor, Senior Moderator
Talanta
. 2023 Oct 24;268(Pt 1):125337.
doi: 10.1016/j.talanta.2023.125337. Online ahead of print. Disposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2
Danielly S Rocha[SUP] 1 [/SUP], Thaísa A Baldo[SUP] 1 [/SUP], Habdias A Silva-Neto[SUP] 1 [/SUP], Gerson F Duarte-Junior[SUP] 1 [/SUP], Gabriela S Bazílio[SUP] 2 [/SUP], Clayton L Borges[SUP] 2 [/SUP], Juliana A Parente-Rocha[SUP] 2 [/SUP], William R de Araujo[SUP] 3 [/SUP], Abner de Siervo[SUP] 4 [/SUP], Thiago L R C Paixão[SUP] 5 [/SUP], Wendell K T Coltro[SUP] 6 [/SUP]
Affiliations
This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protective layer. The COVID-19 diagnostic was based on rapid square wave voltammetry measurements (15 min) using [Fe(CN)[SUB]6[/SUB]][SUP]3-/4-[/SUP] as a redox probe. The method presented a linear response in the concentration range from 250 pg mL[SUP]-1[/SUP] to 20 μg mL[SUP]-1[/SUP] S protein, with a limit of detection of 36.3 pg mL[SUP]-1[/SUP]. The proposed immunosensor was stable for up to two weeks when stored at 4 °C and it demonstrated excellent clinical performance in diagnosing COVID-19 when applied to a panel of 44 undiluted swab samples collected from symptomatic patients. In comparison with results obtained through the quantitative reverse transcription polymerase chain reaction method, the proposed immunosensor offered 100 % accuracy, thus emerging as a powerful alternative candidate for routine and decentralized testing, which can be helpful in controlling the COVID-19 outbreak.
Keywords: COVID-19 diagnostics; Chemical sensors; Disposable biosensors; Nasopharyngeal samples; Point-of-care testing; Thermoplastic polymer.
. 2023 Oct 24;268(Pt 1):125337.
doi: 10.1016/j.talanta.2023.125337. Online ahead of print. Disposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2
Danielly S Rocha[SUP] 1 [/SUP], Thaísa A Baldo[SUP] 1 [/SUP], Habdias A Silva-Neto[SUP] 1 [/SUP], Gerson F Duarte-Junior[SUP] 1 [/SUP], Gabriela S Bazílio[SUP] 2 [/SUP], Clayton L Borges[SUP] 2 [/SUP], Juliana A Parente-Rocha[SUP] 2 [/SUP], William R de Araujo[SUP] 3 [/SUP], Abner de Siervo[SUP] 4 [/SUP], Thiago L R C Paixão[SUP] 5 [/SUP], Wendell K T Coltro[SUP] 6 [/SUP]
Affiliations
- PMID: 39491949
- DOI: 10.1016/j.talanta.2023.125337
This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protective layer. The COVID-19 diagnostic was based on rapid square wave voltammetry measurements (15 min) using [Fe(CN)[SUB]6[/SUB]][SUP]3-/4-[/SUP] as a redox probe. The method presented a linear response in the concentration range from 250 pg mL[SUP]-1[/SUP] to 20 μg mL[SUP]-1[/SUP] S protein, with a limit of detection of 36.3 pg mL[SUP]-1[/SUP]. The proposed immunosensor was stable for up to two weeks when stored at 4 °C and it demonstrated excellent clinical performance in diagnosing COVID-19 when applied to a panel of 44 undiluted swab samples collected from symptomatic patients. In comparison with results obtained through the quantitative reverse transcription polymerase chain reaction method, the proposed immunosensor offered 100 % accuracy, thus emerging as a powerful alternative candidate for routine and decentralized testing, which can be helpful in controlling the COVID-19 outbreak.
Keywords: COVID-19 diagnostics; Chemical sensors; Disposable biosensors; Nasopharyngeal samples; Point-of-care testing; Thermoplastic polymer.