tetano
Editor, Senior Moderator
Talanta
. 2022 Feb 8;242:123297.
doi: 10.1016/j.talanta.2022.123297. Online ahead of print.
Isothermal gene amplification coupled MALDI-TOF MS for SARS-CoV-2 detection
Guobin Han[SUP] 1 [/SUP], Qiuyuan Lin[SUP] 1 [/SUP], Jia Yi[SUP] 1 [/SUP], Qian Lyu[SUP] 2 [/SUP], Qingwei Ma[SUP] 2 [/SUP], Liang Qiao[SUP] 3 [/SUP]
Affiliations
Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide for more than a year and has undergone several mutations and evolutions. Due to the lack of effective therapeutics and long-active vaccines, accurate and large-scale screening and early diagnosis of infected individuals are crucial to control the pandemic. Nevertheless, the current widely used RT-qPCR-based methods suffer from complicated temperature control, long processing time and the risk of false-negative results. Herein, we present a three-way junction induced exponential rolling circle amplification (3WJ-eRCA) combined MALDI-TOF MS assay for SARS-CoV-2 detection. The assay can detect simultaneously the target nucleocapsid (N) and open reading frame 1 ab (orf1ab) genes of SARS-CoV-2 in a single test within 30 min, with an isothermal process (55 °C). High specificity to discriminate SARS-CoV-2 from other coronaviruses, like SARS-CoV, MERS-CoV and bat SARS-like coronavirus (bat-SL-CoVZC45), was observed. We have further used the method to detect pseudovirus of SARS-CoV-2 in various matrices, e.g. water, saliva and urine. The results demonstrated a great potential of the method for large scale screening of COVID-19, which is an important part of the pandemic control.
Keywords: Gene detection; MALDI-TOF MS; Rolling circle amplification; SARS-CoV-2; Virus analysis.
. 2022 Feb 8;242:123297.
doi: 10.1016/j.talanta.2022.123297. Online ahead of print.
Isothermal gene amplification coupled MALDI-TOF MS for SARS-CoV-2 detection
Guobin Han[SUP] 1 [/SUP], Qiuyuan Lin[SUP] 1 [/SUP], Jia Yi[SUP] 1 [/SUP], Qian Lyu[SUP] 2 [/SUP], Qingwei Ma[SUP] 2 [/SUP], Liang Qiao[SUP] 3 [/SUP]
Affiliations
- PMID: 35151081
- DOI: 10.1016/j.talanta.2022.123297
Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide for more than a year and has undergone several mutations and evolutions. Due to the lack of effective therapeutics and long-active vaccines, accurate and large-scale screening and early diagnosis of infected individuals are crucial to control the pandemic. Nevertheless, the current widely used RT-qPCR-based methods suffer from complicated temperature control, long processing time and the risk of false-negative results. Herein, we present a three-way junction induced exponential rolling circle amplification (3WJ-eRCA) combined MALDI-TOF MS assay for SARS-CoV-2 detection. The assay can detect simultaneously the target nucleocapsid (N) and open reading frame 1 ab (orf1ab) genes of SARS-CoV-2 in a single test within 30 min, with an isothermal process (55 °C). High specificity to discriminate SARS-CoV-2 from other coronaviruses, like SARS-CoV, MERS-CoV and bat SARS-like coronavirus (bat-SL-CoVZC45), was observed. We have further used the method to detect pseudovirus of SARS-CoV-2 in various matrices, e.g. water, saliva and urine. The results demonstrated a great potential of the method for large scale screening of COVID-19, which is an important part of the pandemic control.
Keywords: Gene detection; MALDI-TOF MS; Rolling circle amplification; SARS-CoV-2; Virus analysis.