tetano
Editor, Senior Moderator
Talanta
. 2024 Jul 14:279:126572.
doi: 10.1016/j.talanta.2024.126572. Online ahead of print. Ultra-sensitive detection of SARS-CoV-2 S1 protein by coupling rolling circle amplification with poly(N-isopropylacrylamide)-based sandwich-type assay
Yu Feng[SUP] 1 [/SUP], Kebing Yi[SUP] 1 [/SUP], Feng Gong[SUP] 1 [/SUP], Yaran Zhang[SUP] 1 [/SUP], Xiaoyun Shan[SUP] 1 [/SUP], Xinghu Ji[SUP] 2 [/SUP], Fuxiang Zhou[SUP] 3 [/SUP], Zhike He[SUP] 4 [/SUP]
Affiliations
In the past few years, the COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) seriously threatens global public health security due to its high contagiousness. It remains of vital importance to develop a rapid and sensitive assay for SARS-CoV-2. In this work, we proposed a sandwich-type assay based on poly(N-isopropylacrylamide) (PNIPAM), allowing efficient detection of the SARS-CoV-2 S1 protein in the homogeneous solution. Firstly, a direct sandwich-type assay was established with a linear range of 0.2-2 μg/mL and a limit of detection (LOD) of 0.11 μg/mL, which could realize rapid detection in about 1 h. Furthermore, the sandwich-type assay coupled with rolling circle amplification (RCA) obtained an increase in sensitivity of 5.9 × 10[SUP]4[/SUP] folds with a wide linear range of 0.01 - 100 ng/mL and a LOD of 1.88 pg/mL. The average recoveries in unpretreated saliva were 90 %-113.0 %, indicating the potential of the developed method for application in practical samples. Given the high selectivity and sensitivity of the developed method, it has a significant potential for rapid and early detection of SARS-CoV-2.
Keywords: Aptamer; Poly(N-isopropylacrylamide); Rolling circle amplification; SARS-CoV-2.
. 2024 Jul 14:279:126572.
doi: 10.1016/j.talanta.2024.126572. Online ahead of print. Ultra-sensitive detection of SARS-CoV-2 S1 protein by coupling rolling circle amplification with poly(N-isopropylacrylamide)-based sandwich-type assay
Yu Feng[SUP] 1 [/SUP], Kebing Yi[SUP] 1 [/SUP], Feng Gong[SUP] 1 [/SUP], Yaran Zhang[SUP] 1 [/SUP], Xiaoyun Shan[SUP] 1 [/SUP], Xinghu Ji[SUP] 2 [/SUP], Fuxiang Zhou[SUP] 3 [/SUP], Zhike He[SUP] 4 [/SUP]
Affiliations
- PMID: 39024855
- DOI: 10.1016/j.talanta.2024.126572
In the past few years, the COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) seriously threatens global public health security due to its high contagiousness. It remains of vital importance to develop a rapid and sensitive assay for SARS-CoV-2. In this work, we proposed a sandwich-type assay based on poly(N-isopropylacrylamide) (PNIPAM), allowing efficient detection of the SARS-CoV-2 S1 protein in the homogeneous solution. Firstly, a direct sandwich-type assay was established with a linear range of 0.2-2 μg/mL and a limit of detection (LOD) of 0.11 μg/mL, which could realize rapid detection in about 1 h. Furthermore, the sandwich-type assay coupled with rolling circle amplification (RCA) obtained an increase in sensitivity of 5.9 × 10[SUP]4[/SUP] folds with a wide linear range of 0.01 - 100 ng/mL and a LOD of 1.88 pg/mL. The average recoveries in unpretreated saliva were 90 %-113.0 %, indicating the potential of the developed method for application in practical samples. Given the high selectivity and sensitivity of the developed method, it has a significant potential for rapid and early detection of SARS-CoV-2.
Keywords: Aptamer; Poly(N-isopropylacrylamide); Rolling circle amplification; SARS-CoV-2.