tetano
Editor, Senior Moderator
J Infect Dis. 2016 Jun 28. pii: jiw262. [Epub ahead of print]
[h=1]The generation and protective ability of influenza-specific antibody-dependent cellular cytotoxicity in humans elicited by vaccination, natural infection or experimental challenge.[/h] Jegaskanda S[SUP]1[/SUP], Luke C[SUP]2[/SUP], Hickman HD[SUP]3[/SUP], Sangster MY[SUP]4[/SUP], Wieland-Alter WF[SUP]5[/SUP], McBride JM[SUP]6[/SUP], Yewdell JW[SUP]3[/SUP], Wright PF[SUP]5[/SUP], Treanor J[SUP]4[/SUP], Rosenberger CM[SUP]7[/SUP], Subbarao K[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Non-neutralizing antibodies with antibody-dependent cellular cytotoxicity (ADCC) function may provide some protection from influenza infection. The ability of influenza vaccines to induce ADCC mediating antibodies (ADCC-Abs) in adults and children is unclear.
[h=4]METHODS:[/h] We quantified ADCC-Abs in serum samples from adults who received a dose of monovalent 2009 H1N1 pandemic (H1N1pdm09) inactivated subunit vaccine (ISV), or live-attenuated influenza vaccine (LAIV) or had laboratory-confirmed H1N1pdm09 infection. We also measured ADCC-Abs in children who either received a dose of trivalent seasonal ISV followed by trivalent seasonal LAIV or two doses of LAIV. Finally, we assessed the ability of low and high ADCC-Abs titers to protect adults from experimental challenge with influenza A/Wisconsin/67/131/2005 (H3N2) virus.
[h=4]RESULTS:[/h] Adults and children that received a dose of ISV had a robust increase in ADCC-Ab titers to both recombinant (r)HA protein and homologous virus-infected cells. There was no detectable increase in ADCC-Abs to rHA or virus-infected cells in adults and children that received LAIV. Higher (≥320) pre-existing ADCC-Abs were associated with lower virus replication and a significant reduction in total symptom scores in experimentally infected adults.
[h=4]CONCLUSIONS:[/h] ADCC-Abs increased following experimental influenza virus infection in adults and ISV administration in both children and adults.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PMID: 27354365 DOI: 10.1093/infdis/jiw262
[PubMed - as supplied by publisher]
[h=1]The generation and protective ability of influenza-specific antibody-dependent cellular cytotoxicity in humans elicited by vaccination, natural infection or experimental challenge.[/h] Jegaskanda S[SUP]1[/SUP], Luke C[SUP]2[/SUP], Hickman HD[SUP]3[/SUP], Sangster MY[SUP]4[/SUP], Wieland-Alter WF[SUP]5[/SUP], McBride JM[SUP]6[/SUP], Yewdell JW[SUP]3[/SUP], Wright PF[SUP]5[/SUP], Treanor J[SUP]4[/SUP], Rosenberger CM[SUP]7[/SUP], Subbarao K[SUP]8[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Non-neutralizing antibodies with antibody-dependent cellular cytotoxicity (ADCC) function may provide some protection from influenza infection. The ability of influenza vaccines to induce ADCC mediating antibodies (ADCC-Abs) in adults and children is unclear.
[h=4]METHODS:[/h] We quantified ADCC-Abs in serum samples from adults who received a dose of monovalent 2009 H1N1 pandemic (H1N1pdm09) inactivated subunit vaccine (ISV), or live-attenuated influenza vaccine (LAIV) or had laboratory-confirmed H1N1pdm09 infection. We also measured ADCC-Abs in children who either received a dose of trivalent seasonal ISV followed by trivalent seasonal LAIV or two doses of LAIV. Finally, we assessed the ability of low and high ADCC-Abs titers to protect adults from experimental challenge with influenza A/Wisconsin/67/131/2005 (H3N2) virus.
[h=4]RESULTS:[/h] Adults and children that received a dose of ISV had a robust increase in ADCC-Ab titers to both recombinant (r)HA protein and homologous virus-infected cells. There was no detectable increase in ADCC-Abs to rHA or virus-infected cells in adults and children that received LAIV. Higher (≥320) pre-existing ADCC-Abs were associated with lower virus replication and a significant reduction in total symptom scores in experimentally infected adults.
[h=4]CONCLUSIONS:[/h] ADCC-Abs increased following experimental influenza virus infection in adults and ISV administration in both children and adults.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
PMID: 27354365 DOI: 10.1093/infdis/jiw262
[PubMed - as supplied by publisher]