tetano
Editor, Senior Moderator
J Sep Sci. 2017 Jan 31. doi: 10.1002/jssc.201601379. [Epub ahead of print]
[h=1]Three-dimensional-printed magnetophoretic system for the continuous-flow separation of avian influenza H5N1 viruses.[/h] Wang Y[SUP]1,[/SUP][SUP]2[/SUP], Li Y[SUP]2[/SUP], Wang R[SUP]2[/SUP], Wang M[SUP]3[/SUP], Lin J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] As a result of the low concentration of avian influenza viruses in samples for routine screening, the separation and concentration of these viruses are vital for their sensitive detection. We present a novel three-dimensional-printed magnetophoretic system for the continuous-flow separation of the viruses using aptamer-modified magnetic nanoparticles, a magnetophoretic chip, a magnetic field and a fluidic controller. The magnetic field was designed based on finite element magnetic simulation and developed using neodymium magnets with a maximum intensity of 0.65 T and a gradient of 32 T/m for dragging the nanoparticle-virus complexes. The magnetophoretic chip was designed by SOLIDWORKS and fabricated by a three-dimensional printer with a magnetophoretic channel for the continuous-flow separation of the viruses using phosphate buffered saline as carrier flow. The fluidic controller was developed using a microcontroller and peristaltic pumps to inject the carrier flow and the viruses. The trajectory of the virus-nanoparticle complexes was simulated using COMSOL for optimization of the carrier flow and the magnetic field, respectively. The results showed that the H5N1 viruses could be captured, separated and concentrated using the proposed magnetophoretic system with the separation efficiency up to 88% in a continuous-flow separation time of 2 min for a sample volume of 200 μL. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] avian influenza virus; continuous-flow separation; magnetophoresis; three-dimensional printing
PMID: 28139889 DOI: 10.1002/jssc.201601379
[PubMed - as supplied by publisher]
[h=1]Three-dimensional-printed magnetophoretic system for the continuous-flow separation of avian influenza H5N1 viruses.[/h] Wang Y[SUP]1,[/SUP][SUP]2[/SUP], Li Y[SUP]2[/SUP], Wang R[SUP]2[/SUP], Wang M[SUP]3[/SUP], Lin J[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] As a result of the low concentration of avian influenza viruses in samples for routine screening, the separation and concentration of these viruses are vital for their sensitive detection. We present a novel three-dimensional-printed magnetophoretic system for the continuous-flow separation of the viruses using aptamer-modified magnetic nanoparticles, a magnetophoretic chip, a magnetic field and a fluidic controller. The magnetic field was designed based on finite element magnetic simulation and developed using neodymium magnets with a maximum intensity of 0.65 T and a gradient of 32 T/m for dragging the nanoparticle-virus complexes. The magnetophoretic chip was designed by SOLIDWORKS and fabricated by a three-dimensional printer with a magnetophoretic channel for the continuous-flow separation of the viruses using phosphate buffered saline as carrier flow. The fluidic controller was developed using a microcontroller and peristaltic pumps to inject the carrier flow and the viruses. The trajectory of the virus-nanoparticle complexes was simulated using COMSOL for optimization of the carrier flow and the magnetic field, respectively. The results showed that the H5N1 viruses could be captured, separated and concentrated using the proposed magnetophoretic system with the separation efficiency up to 88% in a continuous-flow separation time of 2 min for a sample volume of 200 μL. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] avian influenza virus; continuous-flow separation; magnetophoresis; three-dimensional printing
PMID: 28139889 DOI: 10.1002/jssc.201601379
[PubMed - as supplied by publisher]