tetano
Editor, Senior Moderator
J Immunol. 2015 Sep 25. pii: 1501702. [Epub ahead of print]
[h=1]Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection.[/h] Pichyangkul S[SUP]1[/SUP], Yongvanitchit K[SUP]2[/SUP], Limsalakpetch A[SUP]2[/SUP], Kum-Arb U[SUP]2[/SUP], Im-Erbsin R[SUP]2[/SUP], Boonnak K[SUP]3[/SUP], Thitithayanont A[SUP]4[/SUP], Jongkaewwattana A[SUP]5[/SUP], Wiboon-Ut S[SUP]4[/SUP], Mongkolsirichaikul D[SUP]2[/SUP], Mahanonda R[SUP]6[/SUP], Spring M[SUP]2[/SUP], Chuang I[SUP]2[/SUP], Mason CJ[SUP]2[/SUP], Saunders DL[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Studies of influenza-specific immune responses in humans have largely assessed systemic responses involving serum Ab and peripheral blood T cell responses. However, recent evidence indicates that tissue-resident memory T (T[SUB]RM[/SUB]) cells play an important role in local murine intrapulmonary immunity. Rhesus monkeys were pulmonary exposed to 2009 pandemic H1N1 virus at days 0 and 28 and immune responses in different tissue compartments were measured. All animals were asymptomatic postinfection. Although only minimal memory immune responses were detected in peripheral blood, a high frequency of influenza nucleoprotein-specific memory T cells was detected in the lung at the "contraction phase," 49-58 d after second virus inoculation. A substantial proportion of lung nucleoprotein-specific memory CD8[SUP]+[/SUP] T cells expressed CD103 and CD69, phenotypic markers of T[SUB]RM[/SUB] cells. Lung CD103[SUP]+[/SUP] and CD103[SUP]-[/SUP] memory CD8[SUP]+[/SUP] T cells expressed similar levels of IFN-γ and IL-2. Unlike memory T cells, spontaneous Ab secreting cells and memory B cells specific to influenza hemagglutinin were primarily observed in the mediastinal lymph nodes. Little difference in systemic and local immune responses against influenza was observed between young adult (6-8 y) and old animals (18-28 y). Using a nonhuman primate model, we revealed substantial induction of local T and B cell responses following 2009 pandemic H1N1 infection. Our study identified a subset of influenza-specific lung memory T cells characterized as T[SUB]RM[/SUB] cells in rhesus monkeys. The rhesus monkey model may be useful to explore the role of T[SUB]RM[/SUB] cells in local tissue protective immunity after rechallenge and vaccination.
Copyright ? 2015 by The American Association of Immunologists, Inc.
PMID: 26408671 [PubMed - as supplied by publisher]
[h=1]Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection.[/h] Pichyangkul S[SUP]1[/SUP], Yongvanitchit K[SUP]2[/SUP], Limsalakpetch A[SUP]2[/SUP], Kum-Arb U[SUP]2[/SUP], Im-Erbsin R[SUP]2[/SUP], Boonnak K[SUP]3[/SUP], Thitithayanont A[SUP]4[/SUP], Jongkaewwattana A[SUP]5[/SUP], Wiboon-Ut S[SUP]4[/SUP], Mongkolsirichaikul D[SUP]2[/SUP], Mahanonda R[SUP]6[/SUP], Spring M[SUP]2[/SUP], Chuang I[SUP]2[/SUP], Mason CJ[SUP]2[/SUP], Saunders DL[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Studies of influenza-specific immune responses in humans have largely assessed systemic responses involving serum Ab and peripheral blood T cell responses. However, recent evidence indicates that tissue-resident memory T (T[SUB]RM[/SUB]) cells play an important role in local murine intrapulmonary immunity. Rhesus monkeys were pulmonary exposed to 2009 pandemic H1N1 virus at days 0 and 28 and immune responses in different tissue compartments were measured. All animals were asymptomatic postinfection. Although only minimal memory immune responses were detected in peripheral blood, a high frequency of influenza nucleoprotein-specific memory T cells was detected in the lung at the "contraction phase," 49-58 d after second virus inoculation. A substantial proportion of lung nucleoprotein-specific memory CD8[SUP]+[/SUP] T cells expressed CD103 and CD69, phenotypic markers of T[SUB]RM[/SUB] cells. Lung CD103[SUP]+[/SUP] and CD103[SUP]-[/SUP] memory CD8[SUP]+[/SUP] T cells expressed similar levels of IFN-γ and IL-2. Unlike memory T cells, spontaneous Ab secreting cells and memory B cells specific to influenza hemagglutinin were primarily observed in the mediastinal lymph nodes. Little difference in systemic and local immune responses against influenza was observed between young adult (6-8 y) and old animals (18-28 y). Using a nonhuman primate model, we revealed substantial induction of local T and B cell responses following 2009 pandemic H1N1 infection. Our study identified a subset of influenza-specific lung memory T cells characterized as T[SUB]RM[/SUB] cells in rhesus monkeys. The rhesus monkey model may be useful to explore the role of T[SUB]RM[/SUB] cells in local tissue protective immunity after rechallenge and vaccination.
Copyright ? 2015 by The American Association of Immunologists, Inc.
PMID: 26408671 [PubMed - as supplied by publisher]