tetano
Editor, Senior Moderator
Transfus Apher Sci
. 2020 Aug 25;102922.
doi: 10.1016/j.transci.2020.102922. Online ahead of print.
SARS-CoV-2 serology and virology trends in donors and recipients of convalescent plasma
Latha Dulipsingh[SUP] 1 [/SUP], Danyal Ibrahim[SUP] 2 [/SUP], Ernst J Schaefer[SUP] 3 [/SUP], Rebecca Crowell[SUP] 4 [/SUP], Margaret R Diffenderfer[SUP] 3 [/SUP], Kendra Williams[SUP] 4 [/SUP], Colleen Lima[SUP] 2 [/SUP], Jessica McKenzie[SUP] 4 [/SUP], Lisa Cook[SUP] 5 [/SUP], Jennifer Puff[SUP] 5 [/SUP], Mary Onoroski[SUP] 6 [/SUP], Dorothy B Wakefield[SUP] 4 [/SUP], Reginald J Eadie[SUP] 2 [/SUP], Steven B Kleiboeker[SUP] 7 [/SUP], Patricia Nabors[SUP] 8 [/SUP], Syed A Hussain[SUP] 2 [/SUP]
Affiliations
Abstract
SARS-CoV-2 has infected millions worldwide. The virus is novel, and currently there is no approved treatment. Convalescent plasma may offer a treatment option. We evaluated trends of IgM/IgG antibodies/plasma viral load in donors and recipients of convalescent plasma. 114/139 (82 %) donors had positive IgG antibodies. 46/114 donors tested positive a second time by NP swab. Among those retested, the median IgG declined (p < 0.01) between tests. 25/139 donors with confirmed SARS-CoV-2 were negative for IgG antibodies. This suggests that having had the infection does not necessarily convey immunity, or there is a short duration of immunity associated with a decline in antibodies. Plasma viral load obtained on 35/39 plasma recipients showed 22 (62.9 %) had non-detectable levels on average 14.5 days from positive test versus 6.2 days in those with detectable levels (p < 0.01). There was a relationship between IgG and viral load. IgG was higher in those with non-detectable viral loads. There was no relationship between viral load and blood type (p = 0.87) or death (0.80). Recipients with undetectable viral load had higher IgG levels; there was no relationship between viral load, blood type or death.
Keywords: Adults; Antigens; Blood transfusion; COVID-19; COVID-19 serotherapy; Coronavirus; Coronavirus infections; Humans; Immunoglobulin G; Immunoglobulin M; Plasma; SARS virus; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Viral; Viral load.
. 2020 Aug 25;102922.
doi: 10.1016/j.transci.2020.102922. Online ahead of print.
SARS-CoV-2 serology and virology trends in donors and recipients of convalescent plasma
Latha Dulipsingh[SUP] 1 [/SUP], Danyal Ibrahim[SUP] 2 [/SUP], Ernst J Schaefer[SUP] 3 [/SUP], Rebecca Crowell[SUP] 4 [/SUP], Margaret R Diffenderfer[SUP] 3 [/SUP], Kendra Williams[SUP] 4 [/SUP], Colleen Lima[SUP] 2 [/SUP], Jessica McKenzie[SUP] 4 [/SUP], Lisa Cook[SUP] 5 [/SUP], Jennifer Puff[SUP] 5 [/SUP], Mary Onoroski[SUP] 6 [/SUP], Dorothy B Wakefield[SUP] 4 [/SUP], Reginald J Eadie[SUP] 2 [/SUP], Steven B Kleiboeker[SUP] 7 [/SUP], Patricia Nabors[SUP] 8 [/SUP], Syed A Hussain[SUP] 2 [/SUP]
Affiliations
- PMID: 32883593
- DOI: 10.1016/j.transci.2020.102922
Abstract
SARS-CoV-2 has infected millions worldwide. The virus is novel, and currently there is no approved treatment. Convalescent plasma may offer a treatment option. We evaluated trends of IgM/IgG antibodies/plasma viral load in donors and recipients of convalescent plasma. 114/139 (82 %) donors had positive IgG antibodies. 46/114 donors tested positive a second time by NP swab. Among those retested, the median IgG declined (p < 0.01) between tests. 25/139 donors with confirmed SARS-CoV-2 were negative for IgG antibodies. This suggests that having had the infection does not necessarily convey immunity, or there is a short duration of immunity associated with a decline in antibodies. Plasma viral load obtained on 35/39 plasma recipients showed 22 (62.9 %) had non-detectable levels on average 14.5 days from positive test versus 6.2 days in those with detectable levels (p < 0.01). There was a relationship between IgG and viral load. IgG was higher in those with non-detectable viral loads. There was no relationship between viral load and blood type (p = 0.87) or death (0.80). Recipients with undetectable viral load had higher IgG levels; there was no relationship between viral load, blood type or death.
Keywords: Adults; Antigens; Blood transfusion; COVID-19; COVID-19 serotherapy; Coronavirus; Coronavirus infections; Humans; Immunoglobulin G; Immunoglobulin M; Plasma; SARS virus; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Viral; Viral load.