tetano
Editor, Senior Moderator
Transplant Proc
. 2022 Mar 16;S0041-1345(22)00167-1.
doi: 10.1016/j.transproceed.2022.02.016. Online ahead of print.
Severe Acute Respiratory Syndrome Coronavirus 2 Cross-Reactive B and T Cell Responses in Kidney Transplant Patients
Krystallenia Paniskaki[SUP] 1 [/SUP], Moritz Anft[SUP] 2 [/SUP], Constantin J Thieme[SUP] 3 [/SUP], Sarah Skrzypczyk[SUP] 2 [/SUP], Margarethe Justine Konik[SUP] 4 [/SUP], Sebastian Dolff[SUP] 4 [/SUP], Timm H Westhoff[SUP] 5 [/SUP], Mikalai Nienen[SUP] 6 [/SUP], Anna Stittrich[SUP] 6 [/SUP], Ulrik Stervbo[SUP] 2 [/SUP], Oliver Witzke[SUP] 4 [/SUP], Guido Heine[SUP] 7 [/SUP], Toralf Roch[SUP] 8 [/SUP], Nina Babel[SUP] 8 [/SUP]
Affiliations
Abstract
Background: Immune responses to seasonal endemic coronaviruses might have a pivotal role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Those SARS-CoV-2-crossreactive T cells were recently described in immunocompetent individuals. Still, data on cross-reactive humoral and cellular immunity in kidney transplant recipients is currently lacking.
Methods: The pre-existing, cross-reactive antibody B and T cell immune responses against SARS-CoV-2 in unexposed adults with kidney transplantation (Tx, n = 14) and without (non-Tx, n = 12) sampled before the pandemic were compared with 22 convalescent patients with COVID-19 (Cp) applying enzyme-linked immunosorbent assay and flow cytometry.
Results: In both unexposed groups, SARS-CoV-2 IgG antibodies were not detectable. Memory B cells binding spike (S) protein SARS-CoV-2 were detected in unexposed individuals (64% among Tx; 50% among non-Tx) and higher frequencies after infection (80% Cp). The numbers of SARS-CoV-2-reactive T cells were comparable between patients who had undergone Tx and those who had not. SARS-CoV-2-reactive follicular T helper cells were present in 61% of the unexposed cohort in both patients who had undergone Tx and those who had not.
Conclusions: Cross-reactive memory B and T cells against SARS-CoV-2 exist also in transplanted adults, suggesting a primed adaptive immunity. The effect on the disease course may depend on the concomitant immunosuppressive drugs.
. 2022 Mar 16;S0041-1345(22)00167-1.
doi: 10.1016/j.transproceed.2022.02.016. Online ahead of print.
Severe Acute Respiratory Syndrome Coronavirus 2 Cross-Reactive B and T Cell Responses in Kidney Transplant Patients
Krystallenia Paniskaki[SUP] 1 [/SUP], Moritz Anft[SUP] 2 [/SUP], Constantin J Thieme[SUP] 3 [/SUP], Sarah Skrzypczyk[SUP] 2 [/SUP], Margarethe Justine Konik[SUP] 4 [/SUP], Sebastian Dolff[SUP] 4 [/SUP], Timm H Westhoff[SUP] 5 [/SUP], Mikalai Nienen[SUP] 6 [/SUP], Anna Stittrich[SUP] 6 [/SUP], Ulrik Stervbo[SUP] 2 [/SUP], Oliver Witzke[SUP] 4 [/SUP], Guido Heine[SUP] 7 [/SUP], Toralf Roch[SUP] 8 [/SUP], Nina Babel[SUP] 8 [/SUP]
Affiliations
- PMID: 35489983
- DOI: 10.1016/j.transproceed.2022.02.016
Abstract
Background: Immune responses to seasonal endemic coronaviruses might have a pivotal role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Those SARS-CoV-2-crossreactive T cells were recently described in immunocompetent individuals. Still, data on cross-reactive humoral and cellular immunity in kidney transplant recipients is currently lacking.
Methods: The pre-existing, cross-reactive antibody B and T cell immune responses against SARS-CoV-2 in unexposed adults with kidney transplantation (Tx, n = 14) and without (non-Tx, n = 12) sampled before the pandemic were compared with 22 convalescent patients with COVID-19 (Cp) applying enzyme-linked immunosorbent assay and flow cytometry.
Results: In both unexposed groups, SARS-CoV-2 IgG antibodies were not detectable. Memory B cells binding spike (S) protein SARS-CoV-2 were detected in unexposed individuals (64% among Tx; 50% among non-Tx) and higher frequencies after infection (80% Cp). The numbers of SARS-CoV-2-reactive T cells were comparable between patients who had undergone Tx and those who had not. SARS-CoV-2-reactive follicular T helper cells were present in 61% of the unexposed cohort in both patients who had undergone Tx and those who had not.
Conclusions: Cross-reactive memory B and T cells against SARS-CoV-2 exist also in transplanted adults, suggesting a primed adaptive immunity. The effect on the disease course may depend on the concomitant immunosuppressive drugs.