tetano
Editor, Senior Moderator
J Virol. 2013 Feb 13. [Epub ahead of print]
TRIM22 Inhibits Influenza A Virus Infection by Targeting the Viral Nucleoprotein for Degradation.
Di Pietro A, Kajaste-Rudnitski A, Oteiza A, Nicora L, Towers GJ, Mechti N, Vicenzi E.
Source
Viral Pathogens and Biosafety Unit, Division of Immunology, Transplantation, and Infectious Diseases, San Raffaele Scientific Institute, 20132 Milan, Italy.
Abstract
Tripartite motif (TRIM) protein superfamily members are emerging as important effectors of the innate immune response against viral infections. In particular, TRIM22 was reported to exert antiviral activity against RNA viruses such as Hepatitis B virus (HBV), Encephalomyocarditis virus (ECMV) and Human Immunodeficiency virus-type 1 (HIV-1). We here demonstrate, for the first time, that TRIM22 is upregulated by Influenza A virus (IAV) infection at both mRNA and protein levels in human alveolar epithelial A549 cells. Conversely, TRIM22 potently restricted IAV replication in that prevention of TRIM22 expression by means of shRNA led to a 10-fold enhancement of IAV replication in these cells. Depletion of TRIM22 also reduced the anti-IAV activity of interferon (IFN)-α suggesting that TRIM22 is an important IFN-stimulated gene required for maximal suppression of IAV by type-I IFN. Furthermore, IAV infectious titer decreased up to 100-fold in Madin Darby canine kidney (MDCK) cells expressing exogenous human TRIM22. Restriction of IAV replication was accounted for by the interaction between TRIM22 and the viral nucleoprotein (NP) resulting in its polyubiquitination and degradation in a proteasome-dependent manner. Thus, TRIM22 represents a novel restriction factor upregulated upon IAV infection and that curtails its replicative capacity in epithelial cells.
PMID:
23408607
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23408607
TRIM22 Inhibits Influenza A Virus Infection by Targeting the Viral Nucleoprotein for Degradation.
Di Pietro A, Kajaste-Rudnitski A, Oteiza A, Nicora L, Towers GJ, Mechti N, Vicenzi E.
Source
Viral Pathogens and Biosafety Unit, Division of Immunology, Transplantation, and Infectious Diseases, San Raffaele Scientific Institute, 20132 Milan, Italy.
Abstract
Tripartite motif (TRIM) protein superfamily members are emerging as important effectors of the innate immune response against viral infections. In particular, TRIM22 was reported to exert antiviral activity against RNA viruses such as Hepatitis B virus (HBV), Encephalomyocarditis virus (ECMV) and Human Immunodeficiency virus-type 1 (HIV-1). We here demonstrate, for the first time, that TRIM22 is upregulated by Influenza A virus (IAV) infection at both mRNA and protein levels in human alveolar epithelial A549 cells. Conversely, TRIM22 potently restricted IAV replication in that prevention of TRIM22 expression by means of shRNA led to a 10-fold enhancement of IAV replication in these cells. Depletion of TRIM22 also reduced the anti-IAV activity of interferon (IFN)-α suggesting that TRIM22 is an important IFN-stimulated gene required for maximal suppression of IAV by type-I IFN. Furthermore, IAV infectious titer decreased up to 100-fold in Madin Darby canine kidney (MDCK) cells expressing exogenous human TRIM22. Restriction of IAV replication was accounted for by the interaction between TRIM22 and the viral nucleoprotein (NP) resulting in its polyubiquitination and degradation in a proteasome-dependent manner. Thus, TRIM22 represents a novel restriction factor upregulated upon IAV infection and that curtails its replicative capacity in epithelial cells.
PMID:
23408607
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23408607