tetano
Editor, Senior Moderator
Front Microbiol. 2012;3:128. Epub 2012 Apr 4.
Tropism of Pandemic 2009 H1N1 Influenza a Virus.
Ramadhany R, Yasugi M, Nakamura S, Daidoji T, Watanabe Y, Takahashi K, Ikuta K, Nakaya T.
Source
International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University Suita, Osaka, Japan.
Abstract
Substitutions at the receptor-binding site of the pandemic H1N1 2009 influenza A virus (H1N1pdm) hemagglutinin (HA) gene may be critical in determining whether a virus binds to human or avian receptors. Previous reports suggest that HA Gly(222) and/or Arg(223) allow viruses to bind preferentially to the α2,3-linked sialic acid found in avian species. We also demonstrated that serial passaging of influenza A virus in embryonated chicken eggs increased viral growth 32- to 64-fold, coincident with the increased prevalence of Gly(222) or Arg(223) in HA protein (Yasugi et al., 2012). In this study, we showed that the minor genotype of α2,3-linkage-tropic viruses in upper airways became dominant after passaging through chicken eggs. Viruses possessing HA containing N125D-Q223R, N125D-D187E-Q223R, K119N-D222G, and K119N-N129S-D222G, were detected in both clinical specimens and egg-passaged samples. These results might suggest that egg-adapted viruses, likely represented by α2,3-linkage-tropic virus, were also present in human upper airways as a minor population and transmitted in humans during the outbreak of H1N1pdm.
PMID:
22493594
[PubMed - in process]
PMCID:
PMC3318191
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22493594
Tropism of Pandemic 2009 H1N1 Influenza a Virus.
Ramadhany R, Yasugi M, Nakamura S, Daidoji T, Watanabe Y, Takahashi K, Ikuta K, Nakaya T.
Source
International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University Suita, Osaka, Japan.
Abstract
Substitutions at the receptor-binding site of the pandemic H1N1 2009 influenza A virus (H1N1pdm) hemagglutinin (HA) gene may be critical in determining whether a virus binds to human or avian receptors. Previous reports suggest that HA Gly(222) and/or Arg(223) allow viruses to bind preferentially to the α2,3-linked sialic acid found in avian species. We also demonstrated that serial passaging of influenza A virus in embryonated chicken eggs increased viral growth 32- to 64-fold, coincident with the increased prevalence of Gly(222) or Arg(223) in HA protein (Yasugi et al., 2012). In this study, we showed that the minor genotype of α2,3-linkage-tropic viruses in upper airways became dominant after passaging through chicken eggs. Viruses possessing HA containing N125D-Q223R, N125D-D187E-Q223R, K119N-D222G, and K119N-N129S-D222G, were detected in both clinical specimens and egg-passaged samples. These results might suggest that egg-adapted viruses, likely represented by α2,3-linkage-tropic virus, were also present in human upper airways as a minor population and transmitted in humans during the outbreak of H1N1pdm.
PMID:
22493594
[PubMed - in process]
PMCID:
PMC3318191
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22493594