Two amino acid residues in ion channel protein M2 and polymerase protein PA contribute to replication difference of H5N1 influenza viruses in mice

[tetano]

Virus Res. 2013 Oct 24. pii: S0168-1702(13)00349-3. doi: 10.1016/j.virusres.2013.10.002. [Epub ahead of print]
Two amino acid residues in ion channel protein M2 and polymerase protein PA contribute to replication difference of H5N1 influenza viruses in mice.
Xu C1, Chen H2, Hu B1, Yang S1, Zhang L1, Huang Q1, Zhang X3.
Author information
Abstract

A/swine/Fujian/1/2001 (FJ1) and A/Duck/Zhejiang/52/2000 (DK52) are H5N1 influenza viruses that are lethal in chickens. However, in mice, FJ1 is highly pathogenic, whereas DK52 cannot replicate at all. In this study, we used reverse genetics to demonstrate that amino acid residues at position 54 of polymerase acidic protein (PA) and position 26 of ion channel protein M2 of FJ1 and DK52 are important determinants for replication in mice. In addition, we prove that M2 and PA proteins contribute to the replication of H5N1 viruses in mice.

Copyright ? 2013 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:

M2, PA, mice, replication

PMID:
24512754
[PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/24512754