tetano
Editor, Senior Moderator
J Virol. 2011 Jul 13. [Epub ahead of print]
Type-1 responses of human Vgamma9Vdelta2 T cells to influenza A viruses.
Qin G, Liu Y, Zheng J, Ng IH, Xiang Z, Lam KT, Mao H, Li H, Peiris JS, Lau YL, Tu W.
Source
Departments of Paediatrics & Adolescent Medicine.
Abstract
γδ T cells are essential constituents of anti-microbial and anti-tumor defense. We have recently reported that phosphoantigen isopentenyl pyrophosphate (IPP)-expanded human Vγ9Vδ2 T cells participated in anti-influenza immunity by efficiently killing both human and avian influenza virus-infected monocyte-derived macrophages (MDMs) in vitro. However, little is known about the non-cytolytic responses and trafficking program of γδ T cells to influenza virus. In this study, we found that Vγ9Vδ2 T cells exhibited a type-1 response in both cytokine and chemokine receptor expression during influenza virus infection, and IPP-expanded cells had a higher capacity to produce IFN-γ. Besides their potent cytolytic activity against pandemic H1N1 virus-infected cells, IPP-activated γδ T cells also had non-cytolytic inhibitory effects on seasonal and pandemic H1N1 viruses via IFN-γ, but had no such effects on avian H5N1 or H9N2 viruses. Avian H5N1 and H9N2 viruses induced significantly higher CCL3, CCL4 and CCL5 production in Vγ9Vδ2 T cells than human seasonal H1N1 virus. CCR5 mediated the migration of Vγ9Vδ2 T cells towards influenza virus-infected cells. Our findings suggest a novel therapeutic strategy of using phosphoantigens to boost the antiviral activities of human Vγ9Vδ2 T cells against influenza virus infection.
PMID:
21752902
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21752902
Type-1 responses of human Vgamma9Vdelta2 T cells to influenza A viruses.
Qin G, Liu Y, Zheng J, Ng IH, Xiang Z, Lam KT, Mao H, Li H, Peiris JS, Lau YL, Tu W.
Source
Departments of Paediatrics & Adolescent Medicine.
Abstract
γδ T cells are essential constituents of anti-microbial and anti-tumor defense. We have recently reported that phosphoantigen isopentenyl pyrophosphate (IPP)-expanded human Vγ9Vδ2 T cells participated in anti-influenza immunity by efficiently killing both human and avian influenza virus-infected monocyte-derived macrophages (MDMs) in vitro. However, little is known about the non-cytolytic responses and trafficking program of γδ T cells to influenza virus. In this study, we found that Vγ9Vδ2 T cells exhibited a type-1 response in both cytokine and chemokine receptor expression during influenza virus infection, and IPP-expanded cells had a higher capacity to produce IFN-γ. Besides their potent cytolytic activity against pandemic H1N1 virus-infected cells, IPP-activated γδ T cells also had non-cytolytic inhibitory effects on seasonal and pandemic H1N1 viruses via IFN-γ, but had no such effects on avian H5N1 or H9N2 viruses. Avian H5N1 and H9N2 viruses induced significantly higher CCL3, CCL4 and CCL5 production in Vγ9Vδ2 T cells than human seasonal H1N1 virus. CCR5 mediated the migration of Vγ9Vδ2 T cells towards influenza virus-infected cells. Our findings suggest a novel therapeutic strategy of using phosphoantigens to boost the antiviral activities of human Vγ9Vδ2 T cells against influenza virus infection.
PMID:
21752902
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21752902