tetano
Editor, Senior Moderator
Viral Immunol. 2012 Apr 18. [Epub ahead of print]
Vaccination with CpG-Adjuvanted Avian Influenza Virosomes Promotes Antiviral Immune Responses and Reduces Virus Shedding in Chickens.
Mallick AI, Kulkarni RR, St Paul M, Parvizi P, Nagy E, Behboudi S, Sharif S.
Source
1 Department of Pathobiology, Ontario Veterinary College, University of Guelph , Guelph, Ontario, Canada .
Abstract
Abstract The use of virosomes as a vaccine platform has proven successful against several viruses. Here we examined the protective efficacy of a virosome-based vaccine consisting of avian influenza virus (AIV) A/Duck/Czech/56/H4N6 in chickens against a homologous AIV challenge. Virosomes adjuvanted with CpG-ODN or recombinant chicken interferon (IFN)-γ significantly reduced virus shedding after virus challenge. Furthermore, immunization with virosomes adjuvanted with CpG-ODN increased hemagglutination inhibition (HI) and virus-specific neutralizing serum antibodies, as well as virus-specific serum IgG and mucosal IgA responses. We also found a significant increase in the expression of type I and II interferon genes in the protected birds following virus challenge. In summary, this study demonstrated the ability of virosomes adjuvanted with CpG-ODN to reduce AIV shedding, and elicit virus-specific protective antibody responses in vaccinated birds.
PMID:
22512311
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22512311
Vaccination with CpG-Adjuvanted Avian Influenza Virosomes Promotes Antiviral Immune Responses and Reduces Virus Shedding in Chickens.
Mallick AI, Kulkarni RR, St Paul M, Parvizi P, Nagy E, Behboudi S, Sharif S.
Source
1 Department of Pathobiology, Ontario Veterinary College, University of Guelph , Guelph, Ontario, Canada .
Abstract
Abstract The use of virosomes as a vaccine platform has proven successful against several viruses. Here we examined the protective efficacy of a virosome-based vaccine consisting of avian influenza virus (AIV) A/Duck/Czech/56/H4N6 in chickens against a homologous AIV challenge. Virosomes adjuvanted with CpG-ODN or recombinant chicken interferon (IFN)-γ significantly reduced virus shedding after virus challenge. Furthermore, immunization with virosomes adjuvanted with CpG-ODN increased hemagglutination inhibition (HI) and virus-specific neutralizing serum antibodies, as well as virus-specific serum IgG and mucosal IgA responses. We also found a significant increase in the expression of type I and II interferon genes in the protected birds following virus challenge. In summary, this study demonstrated the ability of virosomes adjuvanted with CpG-ODN to reduce AIV shedding, and elicit virus-specific protective antibody responses in vaccinated birds.
PMID:
22512311
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22512311