tetano
Editor, Senior Moderator
Vaccine
. 2024 Jan 17:S0264-410X(24)00036-7.
doi: 10.1016/j.vaccine.2024.01.036. Online ahead of print. Protective efficacy of a plant-produced beta variant rSARS-CoV-2 VLP vaccine in golden Syrian hamsters
Yolandy Lemmer[SUP] 1 [/SUP], Ros Chapman[SUP] 2 [/SUP], Celia Abolnik[SUP] 3 [/SUP], Tanja Smith[SUP] 4 [/SUP], Georgia Schäfer[SUP] 5 [/SUP], Tandile Hermanus[SUP] 6 [/SUP], Ilse du Preez[SUP] 7 [/SUP], Kruger Goosen[SUP] 8 [/SUP], Kamogelo M Sepotokele[SUP] 4 [/SUP], Sophette Gers[SUP] 9 [/SUP], Tasnim Suliman[SUP] 10 [/SUP], Wolfgang Preiser[SUP] 11 [/SUP], Megan L Shaw[SUP] 12 [/SUP], Robyn Roth[SUP] 7 [/SUP], Alma Truyts[SUP] 13 [/SUP], John Chipangura[SUP] 14 [/SUP], Martin Magwaza[SUP] 15 [/SUP], Osborn Mahanjana[SUP] 16 [/SUP], Penny L Moore[SUP] 6 [/SUP], Martha M O'Kennedy[SUP] 4 [/SUP]
Affiliations
In the quest for heightened protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, we engineered a prototype vaccine utilizing the plant expression system of Nicotiana benthamiana, to produce a recombinant SARS-CoV-2 virus-like particle (VLP) vaccine presenting the S-protein from the Beta (B.1.351) variant of concern (VOC). This innovative vaccine, formulated with either a squalene oil-in-water emulsion or a synthetic CpG oligodeoxynucleotide adjuvant, demonstrated efficacy in a golden Syrian Hamster challenge model. The Beta VLP vaccine induced a robust humoral immune response, with serum exhibiting neutralization not only against SARS-CoV-2 Beta but also cross-neutralizing Delta and Omicron pseudoviruses. Protective efficacy was demonstrated, evidenced by reduced viral RNA copies and mitigated weight loss and lung damage compared to controls. This compelling data instills confidence in the creation of a versatile platform for the local manufacturing of potential pan-sarbecovirus vaccines, against evolving viral threats.
Keywords: Efficacy; Immunogenicity; Pan-sarbecovirus; Virus-like particles.
. 2024 Jan 17:S0264-410X(24)00036-7.
doi: 10.1016/j.vaccine.2024.01.036. Online ahead of print. Protective efficacy of a plant-produced beta variant rSARS-CoV-2 VLP vaccine in golden Syrian hamsters
Yolandy Lemmer[SUP] 1 [/SUP], Ros Chapman[SUP] 2 [/SUP], Celia Abolnik[SUP] 3 [/SUP], Tanja Smith[SUP] 4 [/SUP], Georgia Schäfer[SUP] 5 [/SUP], Tandile Hermanus[SUP] 6 [/SUP], Ilse du Preez[SUP] 7 [/SUP], Kruger Goosen[SUP] 8 [/SUP], Kamogelo M Sepotokele[SUP] 4 [/SUP], Sophette Gers[SUP] 9 [/SUP], Tasnim Suliman[SUP] 10 [/SUP], Wolfgang Preiser[SUP] 11 [/SUP], Megan L Shaw[SUP] 12 [/SUP], Robyn Roth[SUP] 7 [/SUP], Alma Truyts[SUP] 13 [/SUP], John Chipangura[SUP] 14 [/SUP], Martin Magwaza[SUP] 15 [/SUP], Osborn Mahanjana[SUP] 16 [/SUP], Penny L Moore[SUP] 6 [/SUP], Martha M O'Kennedy[SUP] 4 [/SUP]
Affiliations
- PMID: 38238112
- DOI: 10.1016/j.vaccine.2024.01.036
In the quest for heightened protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, we engineered a prototype vaccine utilizing the plant expression system of Nicotiana benthamiana, to produce a recombinant SARS-CoV-2 virus-like particle (VLP) vaccine presenting the S-protein from the Beta (B.1.351) variant of concern (VOC). This innovative vaccine, formulated with either a squalene oil-in-water emulsion or a synthetic CpG oligodeoxynucleotide adjuvant, demonstrated efficacy in a golden Syrian Hamster challenge model. The Beta VLP vaccine induced a robust humoral immune response, with serum exhibiting neutralization not only against SARS-CoV-2 Beta but also cross-neutralizing Delta and Omicron pseudoviruses. Protective efficacy was demonstrated, evidenced by reduced viral RNA copies and mitigated weight loss and lung damage compared to controls. This compelling data instills confidence in the creation of a versatile platform for the local manufacturing of potential pan-sarbecovirus vaccines, against evolving viral threats.
Keywords: Efficacy; Immunogenicity; Pan-sarbecovirus; Virus-like particles.