tetano
Editor, Senior Moderator
Vet Pathol
. 2021 Dec 27;3009858211066841.
doi: 10.1177/03009858211066841. Online ahead of print.
Chronological brain lesions after SARS-CoV-2 infection in hACE2-transgenic mice
Enric Vidal[SUP] 1 [/SUP], Carlos López-Figueroa[SUP] 1 [/SUP], Jordi Rodon[SUP] 1 [/SUP], Mónica Pérez[SUP] 1 [/SUP], Marco Brustolin[SUP] 1 [/SUP], Guillermo Cantero[SUP] 1 [/SUP], Víctor Guallar[SUP] 2 3 [/SUP], Nuria Izquierdo-Useros[SUP] 4 5 [/SUP], Jorge Carrillo[SUP] 4 [/SUP], Julià Blanco[SUP] 4 5 6 [/SUP], Bonaventura Clotet[SUP] 4 5 6 [/SUP], Júlia Vergara-Alert[SUP] 1 [/SUP], Joaquim Segalés[SUP] 7 8 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes respiratory disease, but it can also affect other organs including the central nervous system. Several animal models have been developed to address different key questions related to Coronavirus Disease 2019 (COVID-19). Wild-type mice are minimally susceptible to certain SARS-CoV-2 lineages (beta and gamma variants), whereas hACE2-transgenic mice succumb to SARS-CoV-2 and develop a fatal neurological disease. In this article, we aimed to chronologically characterize SARS-CoV-2 neuroinvasion and neuropathology. Necropsies were performed at different time points, and the brain and olfactory mucosa were processed for histopathological analysis. SARS-CoV-2 virological assays including immunohistochemistry were performed along with a panel of antibodies to assess neuroinflammation. At 6 to 7 days post inoculation (dpi), brain lesions were characterized by nonsuppurative meningoencephalitis and diffuse astrogliosis and microgliosis. Vasculitis and thrombosis were also present and associated with occasional microhemorrhages and spongiosis. Moreover, there was vacuolar degeneration of virus-infected neurons. At 2 dpi, SARS-CoV-2 immunolabeling was only found in the olfactory mucosa, but at 4 dpi intraneuronal virus immunolabeling had already reached most of the brain areas. Maximal distribution of the virus was observed throughout the brain at 6 to 7 dpi except for the cerebellum, which was mostly spared. Our results suggest an early entry of the virus through the olfactory mucosa and a rapid interneuronal spread of the virus leading to acute encephalitis and neuronal damage in this mouse model.
Keywords: Coronavirus Disease 2019; SARS-CoV-2; animal model; brain; hACE2-transgenic mice; meningoencephalitis; neuropathology.
. 2021 Dec 27;3009858211066841.
doi: 10.1177/03009858211066841. Online ahead of print.
Chronological brain lesions after SARS-CoV-2 infection in hACE2-transgenic mice
Enric Vidal[SUP] 1 [/SUP], Carlos López-Figueroa[SUP] 1 [/SUP], Jordi Rodon[SUP] 1 [/SUP], Mónica Pérez[SUP] 1 [/SUP], Marco Brustolin[SUP] 1 [/SUP], Guillermo Cantero[SUP] 1 [/SUP], Víctor Guallar[SUP] 2 3 [/SUP], Nuria Izquierdo-Useros[SUP] 4 5 [/SUP], Jorge Carrillo[SUP] 4 [/SUP], Julià Blanco[SUP] 4 5 6 [/SUP], Bonaventura Clotet[SUP] 4 5 6 [/SUP], Júlia Vergara-Alert[SUP] 1 [/SUP], Joaquim Segalés[SUP] 7 8 [/SUP]
Affiliations
- PMID: 34955064
- DOI: 10.1177/03009858211066841
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes respiratory disease, but it can also affect other organs including the central nervous system. Several animal models have been developed to address different key questions related to Coronavirus Disease 2019 (COVID-19). Wild-type mice are minimally susceptible to certain SARS-CoV-2 lineages (beta and gamma variants), whereas hACE2-transgenic mice succumb to SARS-CoV-2 and develop a fatal neurological disease. In this article, we aimed to chronologically characterize SARS-CoV-2 neuroinvasion and neuropathology. Necropsies were performed at different time points, and the brain and olfactory mucosa were processed for histopathological analysis. SARS-CoV-2 virological assays including immunohistochemistry were performed along with a panel of antibodies to assess neuroinflammation. At 6 to 7 days post inoculation (dpi), brain lesions were characterized by nonsuppurative meningoencephalitis and diffuse astrogliosis and microgliosis. Vasculitis and thrombosis were also present and associated with occasional microhemorrhages and spongiosis. Moreover, there was vacuolar degeneration of virus-infected neurons. At 2 dpi, SARS-CoV-2 immunolabeling was only found in the olfactory mucosa, but at 4 dpi intraneuronal virus immunolabeling had already reached most of the brain areas. Maximal distribution of the virus was observed throughout the brain at 6 to 7 dpi except for the cerebellum, which was mostly spared. Our results suggest an early entry of the virus through the olfactory mucosa and a rapid interneuronal spread of the virus leading to acute encephalitis and neuronal damage in this mouse model.
Keywords: Coronavirus Disease 2019; SARS-CoV-2; animal model; brain; hACE2-transgenic mice; meningoencephalitis; neuropathology.