tetano
Editor, Senior Moderator
Viral Immunol
. 2022 Mar 15.
doi: 10.1089/vim.2021.0168. Online ahead of print.
Correlation of COVID-19 Severity and Immunoglobulin Presence Against Spike and Nucleocapsid Proteins in SARS-CoV-2
Akane Takamatsu[SUP] 1 2 [/SUP], Satoshi Oshiro[SUP] 2 [/SUP], Naeko Mizutani[SUP] 2 [/SUP], Tatsuya Tada[SUP] 2 [/SUP], Yoko Tabe[SUP] 3 [/SUP], Takashi Miida[SUP] 3 [/SUP], Teruo Kirikae[SUP] 2 [/SUP], Yasuaki Tagashira[SUP] 2 4 [/SUP]
Affiliations
Abstract
Data on the human immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins have been applied to vaccine development and diagnosing coronavirus disease 2019 (COVID-19), but little research has been done on the relationship between the human immune response and COVID-19 severity. We herein sought to determine whether there is a correlation between the immunoglobulin level and COVID-19 severity. Clinical samples were collected from 102 patients with COVID-19. Of these, 65 and 37 patients had mild and severe symptoms, respectively. An enzyme-linked immunosorbent assay using the recombinant SARS-CoV-2 nucleocapsid (N) protein, spike (S) protein, and synthetic peptides covering N and S as antigens was performed to measure the IgM and IgG levels. The correlation between the immunoglobulin level and COVID-19 severity was then analyzed. A significant difference in the level of IgG antibodies against N and of IgM antibodies against the receptor binding domain of the S protein was observed between patients with nonsevere and severe COVID-19 symptoms, and the level of IgG antibodies against N was found to be higher in patients with severe symptoms whereas the level of IgM antibodies against the S peptides was higher in patients with nonsevere symptoms. The level of specific antibodies against SARS-CoV-2 structural proteins might correlate with COVID-19 severity. If so, this fact may be useful for predicting the prognosis of the disease and in determining the appropriate treatment with greater precision.
Keywords: COVID-19; ELISA; SARS-CoV-2; recombinant receptor-biding domain; serology.
. 2022 Mar 15.
doi: 10.1089/vim.2021.0168. Online ahead of print.
Correlation of COVID-19 Severity and Immunoglobulin Presence Against Spike and Nucleocapsid Proteins in SARS-CoV-2
Akane Takamatsu[SUP] 1 2 [/SUP], Satoshi Oshiro[SUP] 2 [/SUP], Naeko Mizutani[SUP] 2 [/SUP], Tatsuya Tada[SUP] 2 [/SUP], Yoko Tabe[SUP] 3 [/SUP], Takashi Miida[SUP] 3 [/SUP], Teruo Kirikae[SUP] 2 [/SUP], Yasuaki Tagashira[SUP] 2 4 [/SUP]
Affiliations
- PMID: 35290756
- DOI: 10.1089/vim.2021.0168
Abstract
Data on the human immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins have been applied to vaccine development and diagnosing coronavirus disease 2019 (COVID-19), but little research has been done on the relationship between the human immune response and COVID-19 severity. We herein sought to determine whether there is a correlation between the immunoglobulin level and COVID-19 severity. Clinical samples were collected from 102 patients with COVID-19. Of these, 65 and 37 patients had mild and severe symptoms, respectively. An enzyme-linked immunosorbent assay using the recombinant SARS-CoV-2 nucleocapsid (N) protein, spike (S) protein, and synthetic peptides covering N and S as antigens was performed to measure the IgM and IgG levels. The correlation between the immunoglobulin level and COVID-19 severity was then analyzed. A significant difference in the level of IgG antibodies against N and of IgM antibodies against the receptor binding domain of the S protein was observed between patients with nonsevere and severe COVID-19 symptoms, and the level of IgG antibodies against N was found to be higher in patients with severe symptoms whereas the level of IgM antibodies against the S peptides was higher in patients with nonsevere symptoms. The level of specific antibodies against SARS-CoV-2 structural proteins might correlate with COVID-19 severity. If so, this fact may be useful for predicting the prognosis of the disease and in determining the appropriate treatment with greater precision.
Keywords: COVID-19; ELISA; SARS-CoV-2; recombinant receptor-biding domain; serology.