tetano
Editor, Senior Moderator
Virol J
. 2024 Aug 19;21(1):190.
doi: 10.1186/s12985-024-02451-6. Bacterial sepsis causes more dramatic pathogenetic changes in the Th1 pathway than does viral (COVID-19) sepsis: a prospective observational study of whole blood transcriptomes
Arisa Muratsu[SUP] #[/SUP][SUP] 1 [/SUP], Sayaka Oda[SUP] #[/SUP][SUP] 2 3 [/SUP], Shinya Onishi[SUP] #[/SUP][SUP] 1 [/SUP], Jumpei Yoshimura[SUP] #[/SUP][SUP] 1 [/SUP], Hisatake Matsumoto[SUP] 4 [/SUP], Yuki Togami[SUP] 1 [/SUP], Yumi Mitsuyama[SUP] 1 [/SUP], Hiroshi Ito[SUP] 1 [/SUP], Daisuke Okuzaki[SUP] 3 [/SUP], Hiroshi Ogura[SUP] 1 [/SUP], Jun Oda[SUP] 1 [/SUP]
Affiliations
Objectives: This study aimed to comprehensively compare host responses of patients with bacterial sepsis and those with viral (COVID-19) sepsis by analyzing messenger RNA (mRNA) and microRNA (miRNA) profiles to shed light on their distinct pathophysiological mechanisms.
Design: Prospective observational study.
Setting: Whole blood RNA sequencing was used to analyze mRNA and miRNA profiles of patients diagnosed as having bacterial sepsis or viral (COVID-19) sepsis at the Department of Trauma and Emergency Medicine, Osaka University Graduate School of Medicine.
Patients: Twenty-two bacterial sepsis patients, 35 viral (COVID-19) sepsis patients, and 15 healthy subjects admitted to the department were included. We diagnosed bacterial sepsis patients according to the sepsis-3 criterion that the Sequential Organ Failure Assessment score must increase to 2 points or more among patients with suspected infections. Viral (COVID-19) sepsis patients were diagnosed using SARS-CoV-2 RT-PCR testing, and presence of pneumonia was assessed through chest computed tomography scans.
Interventions: None.
Measurements and main results: For RNA sequencing, 14,500 mRNAs, 1121 miRNAs, and 2556 miRNA-targeted mRNAs were available for analysis in the bacterial sepsis patients. Numbers of genes showing upregulated: downregulated gene expression (false discovery rate < 0.05, |log2 fold change| > 1.5) were 256:2887 for mRNA, 53:5 for miRNA, and 49:2507 for miRNA-targeted mRNA. Similarly, in viral (COVID-19) sepsis patients, 14,500 mRNAs, 1121 miRNAs, and 327 miRNA-targeted mRNAs were analyzed, with numbers of genes exhibiting upregulated: downregulated gene expression of 672:1147 for mRNA, 3:4 for miRNA, and 165:162 for miRNA-targeted mRNA. This analysis revealed significant differences in the numbers of upregulated and downregulated genes expressed and pathways between the bacterial sepsis and viral (COVID-19) sepsis patients. Bacterial sepsis patients showed activation of the PD-1 and PD-L1 cancer immunotherapy signaling pathway and concurrent suppression of Th1 signaling.
Conclusion: Our study illuminated distinct molecular variances between bacterial sepsis and viral (COVID-19) sepsis. Bacterial sepsis patients had a greater number of upregulated and downregulated genes and pathways compared to viral (COVID-19) sepsis patients. Especially, bacterial sepsis caused more dramatic pathogenetic changes in the Th1 pathway than did viral (COVID-19) sepsis.
Keywords: Bacterial sepsis; COVID-19; PD-1 and PD-L1 cancer immunotherapy signaling pathways; Th1 signaling; Whole blood transcriptome.
. 2024 Aug 19;21(1):190.
doi: 10.1186/s12985-024-02451-6. Bacterial sepsis causes more dramatic pathogenetic changes in the Th1 pathway than does viral (COVID-19) sepsis: a prospective observational study of whole blood transcriptomes
Arisa Muratsu[SUP] #[/SUP][SUP] 1 [/SUP], Sayaka Oda[SUP] #[/SUP][SUP] 2 3 [/SUP], Shinya Onishi[SUP] #[/SUP][SUP] 1 [/SUP], Jumpei Yoshimura[SUP] #[/SUP][SUP] 1 [/SUP], Hisatake Matsumoto[SUP] 4 [/SUP], Yuki Togami[SUP] 1 [/SUP], Yumi Mitsuyama[SUP] 1 [/SUP], Hiroshi Ito[SUP] 1 [/SUP], Daisuke Okuzaki[SUP] 3 [/SUP], Hiroshi Ogura[SUP] 1 [/SUP], Jun Oda[SUP] 1 [/SUP]
Affiliations
- PMID: 39160575
- DOI: 10.1186/s12985-024-02451-6
Objectives: This study aimed to comprehensively compare host responses of patients with bacterial sepsis and those with viral (COVID-19) sepsis by analyzing messenger RNA (mRNA) and microRNA (miRNA) profiles to shed light on their distinct pathophysiological mechanisms.
Design: Prospective observational study.
Setting: Whole blood RNA sequencing was used to analyze mRNA and miRNA profiles of patients diagnosed as having bacterial sepsis or viral (COVID-19) sepsis at the Department of Trauma and Emergency Medicine, Osaka University Graduate School of Medicine.
Patients: Twenty-two bacterial sepsis patients, 35 viral (COVID-19) sepsis patients, and 15 healthy subjects admitted to the department were included. We diagnosed bacterial sepsis patients according to the sepsis-3 criterion that the Sequential Organ Failure Assessment score must increase to 2 points or more among patients with suspected infections. Viral (COVID-19) sepsis patients were diagnosed using SARS-CoV-2 RT-PCR testing, and presence of pneumonia was assessed through chest computed tomography scans.
Interventions: None.
Measurements and main results: For RNA sequencing, 14,500 mRNAs, 1121 miRNAs, and 2556 miRNA-targeted mRNAs were available for analysis in the bacterial sepsis patients. Numbers of genes showing upregulated: downregulated gene expression (false discovery rate < 0.05, |log2 fold change| > 1.5) were 256:2887 for mRNA, 53:5 for miRNA, and 49:2507 for miRNA-targeted mRNA. Similarly, in viral (COVID-19) sepsis patients, 14,500 mRNAs, 1121 miRNAs, and 327 miRNA-targeted mRNAs were analyzed, with numbers of genes exhibiting upregulated: downregulated gene expression of 672:1147 for mRNA, 3:4 for miRNA, and 165:162 for miRNA-targeted mRNA. This analysis revealed significant differences in the numbers of upregulated and downregulated genes expressed and pathways between the bacterial sepsis and viral (COVID-19) sepsis patients. Bacterial sepsis patients showed activation of the PD-1 and PD-L1 cancer immunotherapy signaling pathway and concurrent suppression of Th1 signaling.
Conclusion: Our study illuminated distinct molecular variances between bacterial sepsis and viral (COVID-19) sepsis. Bacterial sepsis patients had a greater number of upregulated and downregulated genes and pathways compared to viral (COVID-19) sepsis patients. Especially, bacterial sepsis caused more dramatic pathogenetic changes in the Th1 pathway than did viral (COVID-19) sepsis.
Keywords: Bacterial sepsis; COVID-19; PD-1 and PD-L1 cancer immunotherapy signaling pathways; Th1 signaling; Whole blood transcriptome.