tetano
Editor, Senior Moderator
Virology
. 2020 Jul 29;549:1-4.
doi: 10.1016/j.virol.2020.07.006. Online ahead of print.
A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Tao Wu[SUP] 1 [/SUP], Yiyue Ge[SUP] 2 [/SUP], Kangchen Zhao[SUP] 2 [/SUP], Xiaojuan Zhu[SUP] 2 [/SUP], Yin Chen[SUP] 2 [/SUP], Bin Wu[SUP] 2 [/SUP], Fengcai Zhu[SUP] 2 [/SUP], Baoli Zhu[SUP] 3 [/SUP], Lunbiao Cui[SUP] 2 [/SUP]
Affiliations
Abstract
The current outbreak of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported in China firstly. A rapid, highly sensitive, specific, and simple operational method was needed for the detection of SARS-CoV-2. Here, we established a real-time reverse-transcription recombinase-aided amplification assay (RT-RAA) to detect SARS-CoV-2 rapidly. The primers and probe were designed based on the nucleocapsid protein gene (N gene) sequence of SARS-CoV-2. The detection limit was 10 copies per reaction in this assay, which could be conducted within 15 min at a constant temperature (39 ?C), without any cross-reactions with other respiratory tract pathogens, such as other coronaviruses. Furthermore, compared with commercial real-time RT-PCR assay, it showed a kappa value of 0.959 (p < 0.001) from 150 clinical specimens. These results indicated that this real-time RT-RAA assay may be a valuable tool for detecting SARS-CoV-2.
Keywords: Nucleocapsid protein; Real-time RT-PCR; Real-time RT-RAA; SARS-CoV-2.
. 2020 Jul 29;549:1-4.
doi: 10.1016/j.virol.2020.07.006. Online ahead of print.
A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)
Tao Wu[SUP] 1 [/SUP], Yiyue Ge[SUP] 2 [/SUP], Kangchen Zhao[SUP] 2 [/SUP], Xiaojuan Zhu[SUP] 2 [/SUP], Yin Chen[SUP] 2 [/SUP], Bin Wu[SUP] 2 [/SUP], Fengcai Zhu[SUP] 2 [/SUP], Baoli Zhu[SUP] 3 [/SUP], Lunbiao Cui[SUP] 2 [/SUP]
Affiliations
- PMID: 32758712
- DOI: 10.1016/j.virol.2020.07.006
Abstract
The current outbreak of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported in China firstly. A rapid, highly sensitive, specific, and simple operational method was needed for the detection of SARS-CoV-2. Here, we established a real-time reverse-transcription recombinase-aided amplification assay (RT-RAA) to detect SARS-CoV-2 rapidly. The primers and probe were designed based on the nucleocapsid protein gene (N gene) sequence of SARS-CoV-2. The detection limit was 10 copies per reaction in this assay, which could be conducted within 15 min at a constant temperature (39 ?C), without any cross-reactions with other respiratory tract pathogens, such as other coronaviruses. Furthermore, compared with commercial real-time RT-PCR assay, it showed a kappa value of 0.959 (p < 0.001) from 150 clinical specimens. These results indicated that this real-time RT-RAA assay may be a valuable tool for detecting SARS-CoV-2.
Keywords: Nucleocapsid protein; Real-time RT-PCR; Real-time RT-RAA; SARS-CoV-2.