tetano
Editor, Senior Moderator
Virology
. 2021 Apr 6;559:111-119.
doi: 10.1016/j.virol.2021.03.008. Online ahead of print.
Metabolic shifts modulate lung injury caused by infection with H1N1 influenza A virus
Katherine E Nolan[SUP] 1 [/SUP], Lisa A Baer[SUP] 2 [/SUP], Priyanka Karekar[SUP] 2 [/SUP], Andrew M Nelson[SUP] 3 [/SUP], Kristin I Stanford[SUP] 2 [/SUP], Lauren M Doolittle[SUP] 3 [/SUP], Lucia E Rosas[SUP] 1 [/SUP], Judy M Hickman-Davis[SUP] 1 [/SUP], Harpreet Singh[SUP] 2 [/SUP], Ian C Davis[SUP] 4 [/SUP]
Affiliations
Abstract
Influenza A virus (IAV) infection alters lung epithelial cell metabolism in vitro by promoting a glycolytic shift. We hypothesized that this shift benefits the virus rather than the host and that inhibition of glycolysis would improve infection outcomes. A/WSN/33 IAV-inoculated C57BL/6 mice were treated daily from 1 day post-inoculation (d.p.i.) with 2-deoxy-d-glucose (2-DG) to inhibit glycolysis and with the pyruvate dehydrogenase kinase (PDK) inhibitor dichloroacetate (DCA) to promote flux through the TCA cycle. To block OXPHOS, mice were treated every other day from 1 d.p.i. with the Complex I inhibitor rotenone (ROT). 2-DG significantly decreased nocturnal activity, reduced respiratory exchange ratios, worsened hypoxemia, exacerbated lung dysfunction, and increased humoral inflammation at 6 d.p.i. DCA and ROT treatment normalized oxygenation and airway resistance and attenuated IAV-induced pulmonary edema, histopathology, and nitrotyrosine formation. None of the treatments altered viral replication. These data suggest that a shift to glycolysis is host-protective in influenza.
Keywords: Acute respiratory distress syndrome; Metabolism; Nitrotyrosine; Oxidative phosphorylation.
. 2021 Apr 6;559:111-119.
doi: 10.1016/j.virol.2021.03.008. Online ahead of print.
Metabolic shifts modulate lung injury caused by infection with H1N1 influenza A virus
Katherine E Nolan[SUP] 1 [/SUP], Lisa A Baer[SUP] 2 [/SUP], Priyanka Karekar[SUP] 2 [/SUP], Andrew M Nelson[SUP] 3 [/SUP], Kristin I Stanford[SUP] 2 [/SUP], Lauren M Doolittle[SUP] 3 [/SUP], Lucia E Rosas[SUP] 1 [/SUP], Judy M Hickman-Davis[SUP] 1 [/SUP], Harpreet Singh[SUP] 2 [/SUP], Ian C Davis[SUP] 4 [/SUP]
Affiliations
- PMID: 33865074
- DOI: 10.1016/j.virol.2021.03.008
Abstract
Influenza A virus (IAV) infection alters lung epithelial cell metabolism in vitro by promoting a glycolytic shift. We hypothesized that this shift benefits the virus rather than the host and that inhibition of glycolysis would improve infection outcomes. A/WSN/33 IAV-inoculated C57BL/6 mice were treated daily from 1 day post-inoculation (d.p.i.) with 2-deoxy-d-glucose (2-DG) to inhibit glycolysis and with the pyruvate dehydrogenase kinase (PDK) inhibitor dichloroacetate (DCA) to promote flux through the TCA cycle. To block OXPHOS, mice were treated every other day from 1 d.p.i. with the Complex I inhibitor rotenone (ROT). 2-DG significantly decreased nocturnal activity, reduced respiratory exchange ratios, worsened hypoxemia, exacerbated lung dysfunction, and increased humoral inflammation at 6 d.p.i. DCA and ROT treatment normalized oxygenation and airway resistance and attenuated IAV-induced pulmonary edema, histopathology, and nitrotyrosine formation. None of the treatments altered viral replication. These data suggest that a shift to glycolysis is host-protective in influenza.
Keywords: Acute respiratory distress syndrome; Metabolism; Nitrotyrosine; Oxidative phosphorylation.