tetano
Editor, Senior Moderator
Virology
. 2023 Nov 7:589:109927.
doi: 10.1016/j.virol.2023.109927. Online ahead of print. Reassortant H9N2 canine influenza viruses containing the pandemic H1N1/2009 ribonucleoprotein complex circulating in pigs acquired enhanced virulence in mice
Min Zhu[SUP] 1 [/SUP], Hao Zeng[SUP] 2 [/SUP], Jianqiao He[SUP] 2 [/SUP], Yaohui Zhu[SUP] 1 [/SUP], Pingping Wang[SUP] 1 [/SUP], Jianing Guo[SUP] 2 [/SUP], Jinfan Guo[SUP] 2 [/SUP], Huabo Zhou[SUP] 3 [/SUP], Yifeng Qin[SUP] 1 [/SUP], Kang Ouyang[SUP] 1 [/SUP], Zuzhang Wei[SUP] 1 [/SUP], Weijian Huang[SUP] 1 [/SUP], Ying Chen[SUP] 4 [/SUP]
Affiliations
The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study generated 20 reassortant viruses with the introduction of H1N1/2009 internal genes from EA H1N1 virus into H9N2 virus. 12 of these recovered the replication capability both in the lungs and turbinate samples. 10 of 12 obtained PA gene segments from the ribonucleoprotein (RNP) complexes of the EA H1N1 virus, and 3 exhibited extreme virulence. Specially, the combination of PB2, PA and NP genes could overcome the species-specific restriction in human cells. Analysis of the polymerase activities found that introduction of the PA gene resulted in increased polymerase activity. These findings indicated that RNP complexes from EA H1N1 virus could confer an adaptation advantage and high compatibility to avian H9N2 virus. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
Keywords: Dogs; Eurasian avian-like H1; H1N1/2009-Origin ribonucleoprotein complex; H9N2 influenza virus; Virulence.
. 2023 Nov 7:589:109927.
doi: 10.1016/j.virol.2023.109927. Online ahead of print. Reassortant H9N2 canine influenza viruses containing the pandemic H1N1/2009 ribonucleoprotein complex circulating in pigs acquired enhanced virulence in mice
Min Zhu[SUP] 1 [/SUP], Hao Zeng[SUP] 2 [/SUP], Jianqiao He[SUP] 2 [/SUP], Yaohui Zhu[SUP] 1 [/SUP], Pingping Wang[SUP] 1 [/SUP], Jianing Guo[SUP] 2 [/SUP], Jinfan Guo[SUP] 2 [/SUP], Huabo Zhou[SUP] 3 [/SUP], Yifeng Qin[SUP] 1 [/SUP], Kang Ouyang[SUP] 1 [/SUP], Zuzhang Wei[SUP] 1 [/SUP], Weijian Huang[SUP] 1 [/SUP], Ying Chen[SUP] 4 [/SUP]
Affiliations
- PMID: 37951087
- DOI: 10.1016/j.virol.2023.109927
The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study generated 20 reassortant viruses with the introduction of H1N1/2009 internal genes from EA H1N1 virus into H9N2 virus. 12 of these recovered the replication capability both in the lungs and turbinate samples. 10 of 12 obtained PA gene segments from the ribonucleoprotein (RNP) complexes of the EA H1N1 virus, and 3 exhibited extreme virulence. Specially, the combination of PB2, PA and NP genes could overcome the species-specific restriction in human cells. Analysis of the polymerase activities found that introduction of the PA gene resulted in increased polymerase activity. These findings indicated that RNP complexes from EA H1N1 virus could confer an adaptation advantage and high compatibility to avian H9N2 virus. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
Keywords: Dogs; Eurasian avian-like H1; H1N1/2009-Origin ribonucleoprotein complex; H9N2 influenza virus; Virulence.