tetano
Editor, Senior Moderator
Avian Dis. 2018 Dec;62(4):355-363. doi: 10.1637/11934-072118-Reg.1.
[h=1]Virucidal Efficacy of a Quaternary Ammonium Compound with Food Additive-Grade Calcium Hydroxide Toward Avian Influenza Virus and Newcastle Disease Virus on Abiotic Carriers.[/h] Alam MS[SUP]1,[/SUP][SUP]2[/SUP], Takahashi S[SUP]1[/SUP], Ito M[SUP]1[/SUP], Komura M[SUP]1[/SUP], Ono M[SUP]1[/SUP], Daio C[SUP]1[/SUP], Sangsriratanakul N[SUP]1,[/SUP][SUP]2[/SUP], Shoham D[SUP]1,[/SUP][SUP]3[/SUP], Alam J[SUP]4[/SUP], Takehara K[SUP]5,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] in English, Spanish
The virucidal efficacies of a 0.2% food additive-grade calcium hydroxide [FdCa(OH)[SUB]2[/SUB]] solution, a quaternary ammonium compound (QAC) diluted at 1:500 (QACx500), and their mixture [Mix500; FdCa(OH)[SUB]2[/SUB] powder added at a final concentration of 0.2% to QACx500] were investigated as fomites for avian influenza virus (AIV) and Newcastle disease virus (NDV) on abiotic carriers (steel, rubber, and plastic) at two different temperatures (room temperature [RT; 25 ? 2 C] and 2 C). These viruses were seeded on coupons (5 cm?5 cm) of rubber, steel, or plastic with 5% fetal bovine serum. After complete drying, the coupons were covered with the test solutions at RT or 2 C. After fixed incubation periods, viruses were recovered from the coupons and titrated. At RT, Mix500 required a short time (3 min) to inactivate AIV and NDV to effective levels (≥3 log virus reduction) on rubber, steel, and plastic carriers compared with QAC or FdCa(OH)[SUB]2.[/SUB] At low temperature, QACx500 inactivated AIV on steel and plastic carriers to effective levels within 60 min, whereas Mix500 did so within 10 min. QACx500 and FdCa(OH)[SUB]2[/SUB] solutions could inactivate NDV on steel and plastic carriers within 20 and 10 min, respectively, and Mix500 could do so within 3 min. Viruses on the carriers required longer incubation periods for inactivation at 2 C than at 25 C. These results demonstrate desirable synergistic virucidal effects of Mix500 for important poultry viruses on abiotic carriers, while indicating high applicability within poultry farming.
[h=4]KEYWORDS:[/h] Newcastle disease virus; abiotic carrier; avian influenza virus; food additive?grade calcium hydroxide; low temperature; quaternary ammonium compound; synergistic effects; virucides
PMID: 31119919 DOI: 10.1637/11934-072118-Reg.1
[h=1]Virucidal Efficacy of a Quaternary Ammonium Compound with Food Additive-Grade Calcium Hydroxide Toward Avian Influenza Virus and Newcastle Disease Virus on Abiotic Carriers.[/h] Alam MS[SUP]1,[/SUP][SUP]2[/SUP], Takahashi S[SUP]1[/SUP], Ito M[SUP]1[/SUP], Komura M[SUP]1[/SUP], Ono M[SUP]1[/SUP], Daio C[SUP]1[/SUP], Sangsriratanakul N[SUP]1,[/SUP][SUP]2[/SUP], Shoham D[SUP]1,[/SUP][SUP]3[/SUP], Alam J[SUP]4[/SUP], Takehara K[SUP]5,[/SUP][SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] in English, Spanish
The virucidal efficacies of a 0.2% food additive-grade calcium hydroxide [FdCa(OH)[SUB]2[/SUB]] solution, a quaternary ammonium compound (QAC) diluted at 1:500 (QACx500), and their mixture [Mix500; FdCa(OH)[SUB]2[/SUB] powder added at a final concentration of 0.2% to QACx500] were investigated as fomites for avian influenza virus (AIV) and Newcastle disease virus (NDV) on abiotic carriers (steel, rubber, and plastic) at two different temperatures (room temperature [RT; 25 ? 2 C] and 2 C). These viruses were seeded on coupons (5 cm?5 cm) of rubber, steel, or plastic with 5% fetal bovine serum. After complete drying, the coupons were covered with the test solutions at RT or 2 C. After fixed incubation periods, viruses were recovered from the coupons and titrated. At RT, Mix500 required a short time (3 min) to inactivate AIV and NDV to effective levels (≥3 log virus reduction) on rubber, steel, and plastic carriers compared with QAC or FdCa(OH)[SUB]2.[/SUB] At low temperature, QACx500 inactivated AIV on steel and plastic carriers to effective levels within 60 min, whereas Mix500 did so within 10 min. QACx500 and FdCa(OH)[SUB]2[/SUB] solutions could inactivate NDV on steel and plastic carriers within 20 and 10 min, respectively, and Mix500 could do so within 3 min. Viruses on the carriers required longer incubation periods for inactivation at 2 C than at 25 C. These results demonstrate desirable synergistic virucidal effects of Mix500 for important poultry viruses on abiotic carriers, while indicating high applicability within poultry farming.
[h=4]KEYWORDS:[/h] Newcastle disease virus; abiotic carrier; avian influenza virus; food additive?grade calcium hydroxide; low temperature; quaternary ammonium compound; synergistic effects; virucides
PMID: 31119919 DOI: 10.1637/11934-072118-Reg.1