tetano
Editor, Senior Moderator
Virulence
. 2020 Dec;11(1):964-967.
doi: 10.1080/21505594.2020.1798041.
Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
Florian J Mayer[SUP] 1 [/SUP], Franz Ratzinger[SUP] 2 [/SUP], Ralf L J Schmidt[SUP] 1 [/SUP], Georg Greiner[SUP] 1 [/SUP], Olfert Landt[SUP] 3 [/SUP], Alexander Am Ende[SUP] 3 [/SUP], Victor M Corman[SUP] 4 [/SUP], Nicole Perkmann-Nagele[SUP] 1 [/SUP], Thomas Watkins-Riedel[SUP] 1 [/SUP], Dagmar Petermann[SUP] 1 [/SUP], Karoline Abadir[SUP] 5 [/SUP], Josef Zweim?ller-Mayer[SUP] 5 [/SUP], Robert Strassl[SUP] 1 [/SUP]
Affiliations
Abstract
Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
Keywords: COVID-19; Coronavirus; SARS-CoV-2.
. 2020 Dec;11(1):964-967.
doi: 10.1080/21505594.2020.1798041.
Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
Florian J Mayer[SUP] 1 [/SUP], Franz Ratzinger[SUP] 2 [/SUP], Ralf L J Schmidt[SUP] 1 [/SUP], Georg Greiner[SUP] 1 [/SUP], Olfert Landt[SUP] 3 [/SUP], Alexander Am Ende[SUP] 3 [/SUP], Victor M Corman[SUP] 4 [/SUP], Nicole Perkmann-Nagele[SUP] 1 [/SUP], Thomas Watkins-Riedel[SUP] 1 [/SUP], Dagmar Petermann[SUP] 1 [/SUP], Karoline Abadir[SUP] 5 [/SUP], Josef Zweim?ller-Mayer[SUP] 5 [/SUP], Robert Strassl[SUP] 1 [/SUP]
Affiliations
- PMID: 32726172
- DOI: 10.1080/21505594.2020.1798041
Abstract
Currently, testing for coronavirus is performed with time and personnel consuming PCR assays. The aim of this study was to evaluate the sensitivity, specificity and capacity of a fully automated, random access high-throughput real-time PCR-based diagnostic platform for the detection of SARS-CoV-2. The NeuMoDx N96 system displayed an equal or better detection rate for SARS-CoV-2 compared with the LightCycler 480II system and showed a specificity of 100%. The median PCR run time for all 28 PCR runs was 91 (IQR 84-97) minutes. The capacity of the NeuMoDx N96 could easily surpass the capacity of most currently used molecular test systems and significantly reduce the turn-around time.
Keywords: COVID-19; Coronavirus; SARS-CoV-2.