tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2014 Jan 8. [Epub ahead of print]
Virus-like particle vaccine confers protection against a lethal NDV challenge in chickens and allows DIVA strategy.
Park JK, Lee DH, Yuk SS, To EO, Kwon JH, Noh JY, Kim BY, Choi SW, Kang SM, Lee JB, Park SY, Choi IS, Song CS.
Author information
Abstract
In this study, we developed Newcastle disease virus (NDV) virus-like particles (VLPs) expressing NDV fusion (F) protein along with influenza virus matrix1 (M1) protein using the insect cell expression system. Specific pathogen-free chickens were immunized with oil-emulsion NDV VLP vaccines containing increasing dosages of VLPs (0.4, 2, 10, or 50 μg of VLP/0.5mL⋅dose). Three weeks after immunization, the immunogenicity of the NDV VLP vaccines was determined using a commercial ELISA kit, and a lethal challenge using a highly virulent NDV strain was performed to evaluate the protective efficacy of the NDV VLP vaccines. NDV VLP vaccines elicited anti-NDV antibodies and provided protection against a lethal challenge in a dose-dependent manner. Although the VLP vaccines containing 0.4 or 2μg of VLPs failed to achieve high levels of protection, a single immunization with NDV VLP vaccines containing 10 or 50 μg could fully protect chickens from a lethal challenge and greatly reduced challenge virus shedding. Furthermore, we could easily differentiate infected from vaccinated animals (DIVA) using the hemagglutination inhibition (HI) test. These results strongly suggest that utilization of NDV VLP vaccine in poultry species may be a promising strategy for the better control of NDV.
PMID:
24403523
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24403523
Virus-like particle vaccine confers protection against a lethal NDV challenge in chickens and allows DIVA strategy.
Park JK, Lee DH, Yuk SS, To EO, Kwon JH, Noh JY, Kim BY, Choi SW, Kang SM, Lee JB, Park SY, Choi IS, Song CS.
Author information
Abstract
In this study, we developed Newcastle disease virus (NDV) virus-like particles (VLPs) expressing NDV fusion (F) protein along with influenza virus matrix1 (M1) protein using the insect cell expression system. Specific pathogen-free chickens were immunized with oil-emulsion NDV VLP vaccines containing increasing dosages of VLPs (0.4, 2, 10, or 50 μg of VLP/0.5mL⋅dose). Three weeks after immunization, the immunogenicity of the NDV VLP vaccines was determined using a commercial ELISA kit, and a lethal challenge using a highly virulent NDV strain was performed to evaluate the protective efficacy of the NDV VLP vaccines. NDV VLP vaccines elicited anti-NDV antibodies and provided protection against a lethal challenge in a dose-dependent manner. Although the VLP vaccines containing 0.4 or 2μg of VLPs failed to achieve high levels of protection, a single immunization with NDV VLP vaccines containing 10 or 50 μg could fully protect chickens from a lethal challenge and greatly reduced challenge virus shedding. Furthermore, we could easily differentiate infected from vaccinated animals (DIVA) using the hemagglutination inhibition (HI) test. These results strongly suggest that utilization of NDV VLP vaccine in poultry species may be a promising strategy for the better control of NDV.
PMID:
24403523
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24403523