tetano
Editor, Senior Moderator
Virus Res
. 2020 Jul 4;198088.
doi: 10.1016/j.virusres.2020.198088. Online ahead of print.
Pyruvate Affects Inflammatory Responses of Macrophages During Influenza A Virus Infection
Hazar Abusalamah[SUP] 1 [/SUP], Jessica M Reel[SUP] 1 [/SUP], Christopher R Lupfer[SUP] 2 [/SUP]
Affiliations
Abstract
Pyruvate is the end product of glycolysis and transported into the mitochondria for use in the tricarboxylic acid (TCA) cycle. It is also a common additive in cell culture media. We discovered that inclusion of sodium pyruvate in culture media during infection of mouse bone marrow derived macrophages with influenza A virus impaired cytokine production (IL-6, IL-1β, and TNF-α). Sodium pyruvate did not inhibit viral RNA replication. Instead, the addition of sodium pyruvate alters cellular metabolism and diminished mitochondrial reactive oxygen species (ROS) production and lowered immune signaling. Overall, sodium pyruvate affects the immune response produced by macrophages but does not inhibit virus replication.
Keywords: Inflammation; Nod-like receptor (NLR); antioxidant; inflammasome; influenza A virus; pyruvate.
. 2020 Jul 4;198088.
doi: 10.1016/j.virusres.2020.198088. Online ahead of print.
Pyruvate Affects Inflammatory Responses of Macrophages During Influenza A Virus Infection
Hazar Abusalamah[SUP] 1 [/SUP], Jessica M Reel[SUP] 1 [/SUP], Christopher R Lupfer[SUP] 2 [/SUP]
Affiliations
- PMID: 32634445
- DOI: 10.1016/j.virusres.2020.198088
Abstract
Pyruvate is the end product of glycolysis and transported into the mitochondria for use in the tricarboxylic acid (TCA) cycle. It is also a common additive in cell culture media. We discovered that inclusion of sodium pyruvate in culture media during infection of mouse bone marrow derived macrophages with influenza A virus impaired cytokine production (IL-6, IL-1β, and TNF-α). Sodium pyruvate did not inhibit viral RNA replication. Instead, the addition of sodium pyruvate alters cellular metabolism and diminished mitochondrial reactive oxygen species (ROS) production and lowered immune signaling. Overall, sodium pyruvate affects the immune response produced by macrophages but does not inhibit virus replication.
Keywords: Inflammation; Nod-like receptor (NLR); antioxidant; inflammasome; influenza A virus; pyruvate.